Proteomic fingerprints distinguish microglia, bone marrow, and spleen macrophage populations

Yoshimi Enose; Christopher J. Destache; Andrea L. Mack; James R. Anderson; Fred Ullrich; Pawel S. Ciborowski; Howard E. Gendelman

doi:10.1002/glia.20193

Proteomic fingerprints distinguish microglia, bone marrow, and spleen macrophage populations

Yoshimi Enose, Christopher J. Destache, Andrea L. Mack, James R. Anderson, Fred Ullrich, Pawel S. Ciborowski, Howard E. Gendelman

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Mononuclear phagocytes (MP; dendritic cells, monocytes, tissue macrophages, and microglia) maintain tissue homeostasis and provide a first line of defense against invading pathogens. In specific circumstances, MPs also induce inflammatory responses and as such affect disease onset and progression. Despite intensive research into MP biology, little is known of the functional and molecular properties of individual MP subtypes. Using a novel proteomics platform, unique protein patterns and protein identities were observed among populations of spleen and bone marrow macrophages and microglia. Cells were obtained from C57BL/6 mice and were cultivated in macrophage colony-stimulating factor. MP subtypes were indistinguishable by morphological or antigenic criteria. Protein profiling by Surface Enhanced Laser Desorption Ionization-Time of Flight (SELDI-TOF) ProteinChip® assays with weak cationic exchange chips showed unique MP spectral profiles. Corresponding protein fractions were recovered by high performance liquid chromatography and identified by liquid chromatography tandem mass spectrometry. The results provide a unique means to distinguish microglia from other MP subtypes.

Original language	English (US)
Pages (from-to)	161-172
Number of pages	12
Journal	Glia
Volume	51
Issue number	3
DOIs	https://doi.org/10.1002/glia.20193
State	Published - Aug 15 2005

Keywords

Cellular proteins
Macrophages
Microglia
Proteomics

ASJC Scopus subject areas

Neurology
Cellular and Molecular Neuroscience

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10.1002/glia.20193

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title = "Proteomic fingerprints distinguish microglia, bone marrow, and spleen macrophage populations",

abstract = "Mononuclear phagocytes (MP; dendritic cells, monocytes, tissue macrophages, and microglia) maintain tissue homeostasis and provide a first line of defense against invading pathogens. In specific circumstances, MPs also induce inflammatory responses and as such affect disease onset and progression. Despite intensive research into MP biology, little is known of the functional and molecular properties of individual MP subtypes. Using a novel proteomics platform, unique protein patterns and protein identities were observed among populations of spleen and bone marrow macrophages and microglia. Cells were obtained from C57BL/6 mice and were cultivated in macrophage colony-stimulating factor. MP subtypes were indistinguishable by morphological or antigenic criteria. Protein profiling by Surface Enhanced Laser Desorption Ionization-Time of Flight (SELDI-TOF) ProteinChip{\textregistered} assays with weak cationic exchange chips showed unique MP spectral profiles. Corresponding protein fractions were recovered by high performance liquid chromatography and identified by liquid chromatography tandem mass spectrometry. The results provide a unique means to distinguish microglia from other MP subtypes.",

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author = "Yoshimi Enose and Destache, {Christopher J.} and Mack, {Andrea L.} and Anderson, {James R.} and Fred Ullrich and Ciborowski, {Pawel S.} and Gendelman, {Howard E.}",

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AU - Enose, Yoshimi

AU - Destache, Christopher J.

AU - Mack, Andrea L.

AU - Anderson, James R.

AU - Ullrich, Fred

AU - Ciborowski, Pawel S.

AU - Gendelman, Howard E.

PY - 2005/8/15

Y1 - 2005/8/15

N2 - Mononuclear phagocytes (MP; dendritic cells, monocytes, tissue macrophages, and microglia) maintain tissue homeostasis and provide a first line of defense against invading pathogens. In specific circumstances, MPs also induce inflammatory responses and as such affect disease onset and progression. Despite intensive research into MP biology, little is known of the functional and molecular properties of individual MP subtypes. Using a novel proteomics platform, unique protein patterns and protein identities were observed among populations of spleen and bone marrow macrophages and microglia. Cells were obtained from C57BL/6 mice and were cultivated in macrophage colony-stimulating factor. MP subtypes were indistinguishable by morphological or antigenic criteria. Protein profiling by Surface Enhanced Laser Desorption Ionization-Time of Flight (SELDI-TOF) ProteinChip® assays with weak cationic exchange chips showed unique MP spectral profiles. Corresponding protein fractions were recovered by high performance liquid chromatography and identified by liquid chromatography tandem mass spectrometry. The results provide a unique means to distinguish microglia from other MP subtypes.

AB - Mononuclear phagocytes (MP; dendritic cells, monocytes, tissue macrophages, and microglia) maintain tissue homeostasis and provide a first line of defense against invading pathogens. In specific circumstances, MPs also induce inflammatory responses and as such affect disease onset and progression. Despite intensive research into MP biology, little is known of the functional and molecular properties of individual MP subtypes. Using a novel proteomics platform, unique protein patterns and protein identities were observed among populations of spleen and bone marrow macrophages and microglia. Cells were obtained from C57BL/6 mice and were cultivated in macrophage colony-stimulating factor. MP subtypes were indistinguishable by morphological or antigenic criteria. Protein profiling by Surface Enhanced Laser Desorption Ionization-Time of Flight (SELDI-TOF) ProteinChip® assays with weak cationic exchange chips showed unique MP spectral profiles. Corresponding protein fractions were recovered by high performance liquid chromatography and identified by liquid chromatography tandem mass spectrometry. The results provide a unique means to distinguish microglia from other MP subtypes.

KW - Cellular proteins

KW - Macrophages

KW - Microglia

KW - Proteomics

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Proteomic fingerprints distinguish microglia, bone marrow, and spleen macrophage populations

Abstract

Keywords

ASJC Scopus subject areas

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