@article{d51bbf14b3d24b889d0716365ae79351,
title = "Protocol for engineering and validating a synthetic mitochondrial intermembrane bridge in mammalian cells",
abstract = "Membrane contact sites are recognized as critical means of intercompartmental communication. Here, we describe a protocol for engineering and validating a synthetic bridge between the inner and outer mitochondrial membranes to support functioning of the endogenous mitochondrial contact site and cristae organizing system (MICOS). A chimeric protein, MitoT, is stably expressed in cultured mammalian cells to bridge the mitochondrial membranes. This approach can be a valuable tool to study the function of the MICOS complex and associated proteins. For complete details on the use and execution of this protocol, please refer to Viana et al. (2021).",
keywords = "Biotechnology and bioengineering, Cell Biology, Cell Membrane, Cell culture, Cell isolation, Flow Cytometry/Mass Cytometry, Metabolism, Microscopy, Molecular Biology",
author = "Gunjan Purohit and Viana, {Martonio Ponte} and Oleh Khalimonchuk",
note = "Funding Information: We acknowledge help from the Flow Cytometry Service Center and the Morrison Microscopy Core Facility at the University of Nebraska-Lincoln. We thank Dr. Jennifer Fox for critically reading the manuscript. This work was supported, in whole or in part, by NIH grants GM108975 and GM131701-01 to O.K. Conceptualization, M.P.V. and O.K.; investigation, M.P.V.; writing – original draft, G.P. and M.P.V.; writing – reviewing & editing, M.P.V. G.P. and O.K.; funding acquisition, O.K.; supervision, O.K. The authors declare no competing interests. Funding Information: We acknowledge help from the Flow Cytometry Service Center and the Morrison Microscopy Core Facility at the University of Nebraska-Lincoln. We thank Dr. Jennifer Fox for critically reading the manuscript. This work was supported, in whole or in part, by NIH grants GM108975 and GM131701-01 to O.K. Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = sep,
day = "16",
doi = "10.1016/j.xpro.2022.101454",
language = "English (US)",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "3",
}