Pseudomonas aeruginosa induced lung injury model

Varsha Suresh Kumar, Ruxana T. Sadikot, Jeanette E. Purcell, Asrar B. Malik, Yuru Liu

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

In order to study human acute lung injury and pneumonia, it is important to develop animal models to mimic various pathological features of this disease. Here we have developed a mouse lung injury model by intra-tracheal injection of bacteria Pseudomonas aeruginosa (P. aeruginosa or PA). Using this model, we were able to show lung inflammation at the early phase of injury. In addition, alveolar epithelial barrier leakiness was observed by analyzing bronchoalveolar lavage (BAL); and alveolar cell death was observed by Tunel assay using tissue prepared from injured lungs. At a later phase following injury, we observed cell proliferation required for the repair process. The injury was resolved 7 days from the initiation of P. aeruginosa injection. This model mimics the sequential course of lung inflammation, injury and repair during pneumonia. This clinically relevant animal model is suitable for studying pathology, mechanism of repair, following acute lung injury, and also can be used to test potential therapeutic agents for this disease.

Original languageEnglish (US)
Article numbere52044
JournalJournal of Visualized Experiments
Issue number92
DOIs
StatePublished - Oct 29 2014
Externally publishedYes

Keywords

  • Alveoli
  • Immunology
  • Injury
  • Issue 92
  • Lung
  • Mouse model
  • Pneumonia
  • Pseudomonas

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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