TY - JOUR
T1 - Purification and characterization of a maltase from the extremely thermophilic crenarchaeote Sulfolobus solfataricus
AU - Rolfsmeier, M.
AU - Blum, P.
PY - 1995
Y1 - 1995
N2 - A soluble maltase (α-glucosidase) with an apparent subunit mass of 80 kDa was purified to homogeneity from Sulfolobus solfataricus. The enzyme liberates glucose from maltose and malto-oligomers. Maximal activity was observed at 105°C, with half-lives of 11 h (85°C), 3.0 h (95°C), and 2.75 h (100°C). The enzyme was generally resistant to proteolysis and denaturants including aliphatic alcohols. n-Propanol treatment at 85°C increased both K(m) and V(max) for maltose hydrolysis.
AB - A soluble maltase (α-glucosidase) with an apparent subunit mass of 80 kDa was purified to homogeneity from Sulfolobus solfataricus. The enzyme liberates glucose from maltose and malto-oligomers. Maximal activity was observed at 105°C, with half-lives of 11 h (85°C), 3.0 h (95°C), and 2.75 h (100°C). The enzyme was generally resistant to proteolysis and denaturants including aliphatic alcohols. n-Propanol treatment at 85°C increased both K(m) and V(max) for maltose hydrolysis.
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U2 - 10.1128/jb.177.2.482-485.1995
DO - 10.1128/jb.177.2.482-485.1995
M3 - Comment/debate
C2 - 7814342
AN - SCOPUS:0028852767
VL - 177
SP - 482
EP - 485
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 2
ER -