Purification and characterization of a maltase from the extremely thermophilic crenarchaeote Sulfolobus solfataricus

M. Rolfsmeier, P. Blum

Research output: Contribution to journalComment/debate

90 Scopus citations

Abstract

A soluble maltase (α-glucosidase) with an apparent subunit mass of 80 kDa was purified to homogeneity from Sulfolobus solfataricus. The enzyme liberates glucose from maltose and malto-oligomers. Maximal activity was observed at 105°C, with half-lives of 11 h (85°C), 3.0 h (95°C), and 2.75 h (100°C). The enzyme was generally resistant to proteolysis and denaturants including aliphatic alcohols. n-Propanol treatment at 85°C increased both K(m) and V(max) for maltose hydrolysis.

Original languageEnglish (US)
Pages (from-to)482-485
Number of pages4
JournalJournal of bacteriology
Volume177
Issue number2
DOIs
StatePublished - 1995

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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