Purification and characterization of porphyromonas gingivalis outer membrane antigens

Boris Polak; Michael A. Peck; John K. Dyer; Philip S. Bird; Richard A. Reinhardt; Gregory J. Seymour

doi:10.1016/0003-9969(95)00063-U

Purification and characterization of porphyromonas gingivalis outer membrane antigens

Boris Polak, Michael A. Peck, John K. Dyer, Philip S. Bird, Richard A. Reinhardt, Gregory J. Seymour

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Porphyromonas gingivalis is strongly associated with periodontal disease. Significant titres of specific IgG antibodies to P. gingivalis can be found in healthy individuals and those with periodontitis. In this study, 22 outer membrane antigens ranging from 15.5 to 107.6 kDa were recognized by sera from persons with periodontitis and controls. Serum from individuals with periodontitis showed a significantly higher IgG response to a 31.4-kDa antigen (p < 0.05); serum from those with gingivitis demonstrated a significantly higher response to a 15.5-kDa antigen (p < 0.05). The response to the 15.5-kDa antigen might represent a protective immune response while that to the 31.4-kDa could serve as a marker for disease susceptibility. These two antigens were purified to homogeneity and their N-terminal amino acid sequences determined. The sequences did not correspond to any previously described P. gingivalis antigens. The role of these two antigens in the pathogenesis of periodontal disease remains to be determined.

Original language	English (US)
Pages (from-to)	905-912
Number of pages	8
Journal	Archives of Oral Biology
Volume	40
Issue number	10
DOIs	https://doi.org/10.1016/0003-9969(95)00063-U
State	Published - Oct 1995

Keywords

Porphyromonas gingivalis
outer membrane antigens
periodontal disease
purification
western blotting

ASJC Scopus subject areas

Otorhinolaryngology
Dentistry(all)
Cell Biology

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10.1016/0003-9969(95)00063-U

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title = "Purification and characterization of porphyromonas gingivalis outer membrane antigens",

abstract = "Porphyromonas gingivalis is strongly associated with periodontal disease. Significant titres of specific IgG antibodies to P. gingivalis can be found in healthy individuals and those with periodontitis. In this study, 22 outer membrane antigens ranging from 15.5 to 107.6 kDa were recognized by sera from persons with periodontitis and controls. Serum from individuals with periodontitis showed a significantly higher IgG response to a 31.4-kDa antigen (p < 0.05); serum from those with gingivitis demonstrated a significantly higher response to a 15.5-kDa antigen (p < 0.05). The response to the 15.5-kDa antigen might represent a protective immune response while that to the 31.4-kDa could serve as a marker for disease susceptibility. These two antigens were purified to homogeneity and their N-terminal amino acid sequences determined. The sequences did not correspond to any previously described P. gingivalis antigens. The role of these two antigens in the pathogenesis of periodontal disease remains to be determined.",

keywords = "Porphyromonas gingivalis, outer membrane antigens, periodontal disease, purification, western blotting",

author = "Boris Polak and Peck, {Michael A.} and Dyer, {John K.} and Bird, {Philip S.} and Reinhardt, {Richard A.} and Seymour, {Gregory J.}",

note = "Funding Information: Acknowledgements--This study was supported by the National Health and Medical Research Council of Australia and by grants from the Juvenile Diabetes Foundation International No. 189860 and The Colgate Palmolive Company.",

year = "1995",

month = oct,

doi = "10.1016/0003-9969(95)00063-U",

language = "English (US)",

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AU - Peck, Michael A.

AU - Dyer, John K.

AU - Bird, Philip S.

AU - Reinhardt, Richard A.

AU - Seymour, Gregory J.

N1 - Funding Information: Acknowledgements--This study was supported by the National Health and Medical Research Council of Australia and by grants from the Juvenile Diabetes Foundation International No. 189860 and The Colgate Palmolive Company.

PY - 1995/10

Y1 - 1995/10

N2 - Porphyromonas gingivalis is strongly associated with periodontal disease. Significant titres of specific IgG antibodies to P. gingivalis can be found in healthy individuals and those with periodontitis. In this study, 22 outer membrane antigens ranging from 15.5 to 107.6 kDa were recognized by sera from persons with periodontitis and controls. Serum from individuals with periodontitis showed a significantly higher IgG response to a 31.4-kDa antigen (p < 0.05); serum from those with gingivitis demonstrated a significantly higher response to a 15.5-kDa antigen (p < 0.05). The response to the 15.5-kDa antigen might represent a protective immune response while that to the 31.4-kDa could serve as a marker for disease susceptibility. These two antigens were purified to homogeneity and their N-terminal amino acid sequences determined. The sequences did not correspond to any previously described P. gingivalis antigens. The role of these two antigens in the pathogenesis of periodontal disease remains to be determined.

AB - Porphyromonas gingivalis is strongly associated with periodontal disease. Significant titres of specific IgG antibodies to P. gingivalis can be found in healthy individuals and those with periodontitis. In this study, 22 outer membrane antigens ranging from 15.5 to 107.6 kDa were recognized by sera from persons with periodontitis and controls. Serum from individuals with periodontitis showed a significantly higher IgG response to a 31.4-kDa antigen (p < 0.05); serum from those with gingivitis demonstrated a significantly higher response to a 15.5-kDa antigen (p < 0.05). The response to the 15.5-kDa antigen might represent a protective immune response while that to the 31.4-kDa could serve as a marker for disease susceptibility. These two antigens were purified to homogeneity and their N-terminal amino acid sequences determined. The sequences did not correspond to any previously described P. gingivalis antigens. The role of these two antigens in the pathogenesis of periodontal disease remains to be determined.

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KW - periodontal disease

KW - purification

KW - western blotting

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Purification and characterization of porphyromonas gingivalis outer membrane antigens

Abstract

Keywords

ASJC Scopus subject areas

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