Purification and Initial Characterization of Ara h 7, a Peanut Allergen from the 2S Albumin Protein Family

Danijela Apostolovic, Justin T. Marsh, Joe Baumert, Steve L. Taylor, Adrie Westphal, Harmen De Jongh, Phil Johnson, Govardus A.H. De Jong, Stef J. Koppelman

Research output: Contribution to journalArticlepeer-review

Abstract

2S albumins are important peanut allergens. Within this protein family, Ara h 2 and Ara h 6 have been described in detail, but Ara h 7 has received little attention. We now describe the first purification of Ara h 7 and its characterization. Two Ara h 7 isoforms were purified from peanuts. Mass spectrometry revealed that both the isoforms have a post-translation cleavage, a hydroxyproline modification near the N-terminus, and four disulfide bonds. The secondary structure of both Ara h 7 isoforms is highly comparable to those of Ara h 2 and Ara h 6. Both Ara h 7 isoforms bind IgE, and Ara h 7 is capable of inhibiting the binding between Ara h 2 and IgE, suggesting at least partially cross-reactive IgE epitopes. Ara h 7 was found in all main market types of peanut, at comparable levels. This suggests that Ara h 7 is a relevant allergen from the peanut 2S albumin protein family.

Original languageEnglish (US)
Pages (from-to)6318-6329
Number of pages12
JournalJournal of Agricultural and Food Chemistry
Volume69
Issue number22
DOIs
StatePublished - Jun 9 2021

Keywords

  • 2S albumin
  • Ara h 7
  • Arachis hypogaea
  • allergen
  • peanuts

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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