Purification of α-amylase from Bacillus licheniformis by chromatofocusing and gel filtration chromatography

M. Damodara Rao, A. Purnima, D. V. Ramesh, C. Ayyanna

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

A combination of chromatofocusing and gel filtration chromatography resulted in a simple purification of α-amylase from Bacillus licheniformis. The purification was approximately 77-fold. Identification of the purity was established by SDS-PAGE. Molecular weight and isoelectric point of the purified enzyme were 58 kDa and 7.18 respectively. Western blot analysis confirms the specificity of antibody raised against purified α-amylase.

Original languageEnglish (US)
Pages (from-to)547-550
Number of pages4
JournalWorld Journal of Microbiology and Biotechnology
Volume18
Issue number6
DOIs
StatePublished - 2002

Keywords

  • Chromatofocusing
  • Gel filtration chromatography
  • α-Amylase

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

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