Purification of poly-β-hydroxybutyrate by density gradient centrifugation in sodium bromide

K. W. Nickerson

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Fractionation of fully sporulated cultures of Bacillus thuringiensis by density gradient centrifugation in NaBr produced two bands which were identified as poly-β-hydroxybutyrate. This technique generated high yields of membrane-bound and unbound granules of exceptional purity and degree of polymerization.

Original languageEnglish (US)
Pages (from-to)1208-1209
Number of pages2
JournalApplied and environmental microbiology
Volume43
Issue number5
DOIs
StatePublished - 1982
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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