Putative role of the phosphatidylinositol 3-kinase-Akt signaling pathway in the survival of granulosa cells

Suzanne D. Westfall, Isabel R. Hendry, Kevin L. Obholz, Bo R. Rueda, John S. Davis

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Insulin-like growth factor-I (IGF-I) is an important differentiation and survival factor for granulosa cells. The purpose of this study was to test the hypothesis that IGF-I promotes survival of porcine granulosa cells by signaling through the phosphatidylinositol (PI) 3-kinase/Akt signal transduction pathway. Treatment with IGF-I (100 ng/mL) for 10 min stimulated PI 3-kinase and Akt protein kinase activity. IGF-I stimulated the phosphorylation and activation of Akt in a time- and concentration-dependent manner. The PI 3-kinase inhibitors wortmannin and LY294002 blocked IGF-I induced increases in PI 3-kinase activity and phosphorylation of Akt. Additionally, IGF-I treatment prevented apoptosis. The survival response to IGF-I was blocked by treatment with either wortmannin or LY294002. These data suggest that IGF-I-induced phosphorylation of Akt is mediated through PI 3- kinase and that inactivation of this pathway results in granulosa cell apoptosis. We conclude that the PI 3-kinase/Akt signaling serves as a functional survival pathway in the ovary.

Original languageEnglish (US)
Pages (from-to)315-321
Number of pages7
JournalEndocrine
Volume12
Issue number3
StatePublished - 2000

Keywords

  • Akt
  • Apoptosis
  • Granulosa cell
  • Insulin-like growth factor- I
  • Ovary
  • Phosphatidylinositol 3-kinase
  • Signal transduction

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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    Westfall, S. D., Hendry, I. R., Obholz, K. L., Rueda, B. R., & Davis, J. S. (2000). Putative role of the phosphatidylinositol 3-kinase-Akt signaling pathway in the survival of granulosa cells. Endocrine, 12(3), 315-321.