TY - GEN
T1 - Quantification of Cell Adhesion Strength using Energy Dissipation from Quartz Microbalance with Dissipation Monitoring
AU - Esfahani, Amir Monemian
AU - Rosenbohm, Jordan
AU - Yang, Ruiguo
PY - 2019/7
Y1 - 2019/7
N2 - We propose a mechanical model that describes the energy dissipation process in the probing of cell adhesion using quartz crystal microbalance with dissipation monitoring (QCM-D). The model considers the QCM-D disk as a harmonic oscillator and the friction between the disk and the cell is modeled as molecular bond rupturing and the fluidic slip at the interface. The bond formation and rupture events are governed by relative motion between the sensor disk and the cell membrane. We consider this interaction as the main energy dissipation channel for the oscillator, as the dynamic molecular bond rupture and the viscous damping of the trapped liquid at the cell/disk interfacial layer contribute to the most energy loss during the harmonic oscillation. The energy loss due to the frictional slip of the stress fiber/cytoplasm is insignificant compared with the bond rupture. At high bond number conditions, the energy dissipation will be dominated by the bond rupture events at the focal adhesion, and bond number and the size of focal adhesion are linearly related to the energy dissipation factors. These findings can serve as an analytical tool for QCM-D based cell adhesion assays.
AB - We propose a mechanical model that describes the energy dissipation process in the probing of cell adhesion using quartz crystal microbalance with dissipation monitoring (QCM-D). The model considers the QCM-D disk as a harmonic oscillator and the friction between the disk and the cell is modeled as molecular bond rupturing and the fluidic slip at the interface. The bond formation and rupture events are governed by relative motion between the sensor disk and the cell membrane. We consider this interaction as the main energy dissipation channel for the oscillator, as the dynamic molecular bond rupture and the viscous damping of the trapped liquid at the cell/disk interfacial layer contribute to the most energy loss during the harmonic oscillation. The energy loss due to the frictional slip of the stress fiber/cytoplasm is insignificant compared with the bond rupture. At high bond number conditions, the energy dissipation will be dominated by the bond rupture events at the focal adhesion, and bond number and the size of focal adhesion are linearly related to the energy dissipation factors. These findings can serve as an analytical tool for QCM-D based cell adhesion assays.
UR - http://www.scopus.com/inward/record.url?scp=85081065740&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85081065740&partnerID=8YFLogxK
U2 - 10.1109/NANO46743.2019.8993951
DO - 10.1109/NANO46743.2019.8993951
M3 - Conference contribution
AN - SCOPUS:85081065740
T3 - Proceedings of the IEEE Conference on Nanotechnology
SP - 265
EP - 268
BT - 19th IEEE International Conference on Nanotechnology, NANO 2019
PB - IEEE Computer Society
T2 - 19th IEEE International Conference on Nanotechnology, NANO 2019
Y2 - 22 July 2019 through 26 July 2019
ER -