Quantification of nisin in flow-injection immunoassay systems

R. Nandakumar, M. P. Nandakumar, B. Mattiasson

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

A monoclonal-antibody-based, sequential competitive-flow-injection immunoassay system in expanded-bed mode has been developed for the determination of nisin. The system allows the determination of nisin in the presence of suspended particles without any significant interference, illustrating its potential for on-line monitoring of fermentation processes or the analysis of food matrices. The dose response range of the system when operated in expanded-bed mode was 6-90 μM. The detection limit under packed-bed conditions was 3 μM. The results correlated well with the results from conventional ELISA in the analysis of samples of processed cheese. When milk samples, fermentation samples and buffer were spiked with nisin, the mean recoveries were 86% for milk samples, 96% for fermentation samples and 98% for buffer solution. Copyright (C) 2000 Elsevier Science S.A.

Original languageEnglish (US)
Pages (from-to)241-247
Number of pages7
JournalBiosensors and Bioelectronics
Volume15
Issue number5-6
DOIs
StatePublished - Aug 2000

Keywords

  • Expanded bed
  • Flow injection
  • Immunoassay
  • Nisin

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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