We observed and evaluated normal and neoplastic cells of the human bladder by SEM in a preliminary assessment of surface topography with regard to tumor growth, grade, and stage. Data collected so far indicates that changes in the surface topography of the exfoliated cells may possibly vary both in relation to tumor grade and stage. Based on these qualitative observations, we expanded our approach by using a computerized image analysis system directly interfaced with the SEM. The measurements which can be made include cell surface area, diameter, length, width, perimeter, orientation and number of pleomorphic microvilli (PMV) per unit area. Statistical analysis is also performed. The boundaries between cells are not recognizable by the system, making the single cells present in cytologic specimens ideal for evaluation. Uniform short microvilli are readily distinguished from PMV. Preliminary evaluation of 23 patients has distinguished specimens from malignant cases compared to patients with benign lesions.
|Original language||English (US)|
|Number of pages||6|
|Journal||Scanning Electron Microscopy|
|State||Published - Sep 1 1985|
ASJC Scopus subject areas
- Control and Systems Engineering