Quantitative analysis of species specificity of two anti-parvalbumin antibodies for detecting southern hemisphere fish species demonstrating strong phylogenetic association

Ji Liang, Chui Choo Tan, Steve L. Taylor, Joseph L. Baumert, Andreas L. Lopata, N. Alice Lee

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

This study aimed to develop a novel approach to determine the correlation between the parvalbumin (PAV) contents and their corresponding immunoreactivity (detectability) in southern hemisphere fish species. The immuno-detected PAV contents of the test fish species were estimated by a quantitative SDS-PAGE. A quantitative Enzyme-Linked ImmunoSorbent Assay (ELISA) was formatted to assess relative immunoreactivity of PAV. Sixteen species (forty-three percent) displayed a positive correlation with the anti-cod PAV polyclonal antibody, but no correlation with the anti-carp PAV monoclonal antibody. There was a strong phylogenetic association of the PAV immunoreactivity. Species from the order of Perciformes showed strong binding with both antibodies; whereas species from Salmoniformes, Ophidiiformes, Scombriformes, Scorpaeniformes, and Tetraodontiformes showed weak or no binding. This approach showed for the first time a statistical correlation between the PAV content and the immunoreactivity and allowed to rank the relative species/order specificity of the two antibodies for the southern hemisphere fish PAV.

Original languageEnglish (US)
Pages (from-to)588-596
Number of pages9
JournalFood Chemistry
Volume237
DOIs
StatePublished - Dec 15 2017

Keywords

  • Anti-carp parvalbumin antibody
  • Anti-cod parvalbumin antibody
  • ELISA
  • Order specificity
  • Parvalbumin
  • Southern hemisphere fish
  • Species specificity

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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