Quantitative dextran trafficking to the Coxiella burnetii parasitophorous vacuole

Seth Winfree, Stacey D. Gilk

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


The gram-negative bacterium Coxiella burnetii causes humanQfever, a disease characterized by a debilitating flu-like illness in acute cases and endocarditis in chronic patients. An obligate intracellular pathogen, Coxiella burnetii survives within a large, lysosome-like vacuole inside the host cell. A unique feature of the Coxiella parasitophorous vacuole (PV) is high levels of fusion with the host endocytic pathway, with PV-endosome fusion critical for Coxiella survival within the host cell. This unit describes quantitating PV-endosome fusion by measuring delivery of the fluid phase endosome marker dextran to the PV using live cell imaging. To study the effect of host cell proteins involved in PV-endosome fusion, details are provided for using siRNA knockdown host cells. This method is a powerful tool for understanding mechanisms underlying Coxiella’s ability to manipulate host cell trafficking pathways.

Original languageEnglish (US)
Pages (from-to)6C.2.1-6C.2.12
JournalCurrent protocols in microbiology
StatePublished - Aug 1 2017
Externally publishedYes


  • Coxiella burnetii
  • Endocytic trafficking
  • Parasitophorous vacuole
  • Quantitative microscopy

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology


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