TY - JOUR
T1 - Quantitative enzymatic synthesis of 2-acetamido-2-deoxy-α-d-glucopyranosyl-1,6-diphosphate
AU - Cheng, Pi Wan
AU - Carlson, Don M.
N1 - Funding Information:
1 Supported in part by NIH Grant AM-10335. Some of these data have been presented at the 65th Annual Meeting of the American Society of Biological Chemists at Minneapolis, Minnesota, in June 1974. 2 Current address: Department of Pediatrics, University Hospitals, Case Western Reserve University, Cleveland, Ohio 44106. 3 Current address: Department of Biochemistry, Purdue University. West Lafayette, Indiana 47907. To whom correspondence should be sent.
PY - 1978/4
Y1 - 1978/4
N2 - Phosphoacetylglucosamine mutase (EC 2.7.5.2) catalyzes the interconversion of N-acetylglucosamine-1-phosphate and of N-acetylglucosamine-6-phosphate. Glucose-1,6-diphosphate activates this mutase through the formation of N-acetylglucosamine-1,6-diphosphate and glucose-6-phosphate. By adding glucose-6-phosphate dehydrogenase and an excess of N-acetylglucosamine-6-phosphate the reaction is pulled to completion with stoichiometric formation of N-acetylglucosamine-1,6-diphosphate with respect to the amount of glucose-1,6-diphosphate present. The isolation, chemical analysis, molar rotation, and cofactor activity of N-acetylglucosamine-1,6-diphosphate are described.
AB - Phosphoacetylglucosamine mutase (EC 2.7.5.2) catalyzes the interconversion of N-acetylglucosamine-1-phosphate and of N-acetylglucosamine-6-phosphate. Glucose-1,6-diphosphate activates this mutase through the formation of N-acetylglucosamine-1,6-diphosphate and glucose-6-phosphate. By adding glucose-6-phosphate dehydrogenase and an excess of N-acetylglucosamine-6-phosphate the reaction is pulled to completion with stoichiometric formation of N-acetylglucosamine-1,6-diphosphate with respect to the amount of glucose-1,6-diphosphate present. The isolation, chemical analysis, molar rotation, and cofactor activity of N-acetylglucosamine-1,6-diphosphate are described.
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U2 - 10.1016/0003-2697(78)90251-8
DO - 10.1016/0003-2697(78)90251-8
M3 - Article
C2 - 646107
AN - SCOPUS:0018171768
VL - 85
SP - 533
EP - 540
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -