Quantitative real-time PCR expression analysis of peripheral blood mononuclear cells in pancreatic cancer patients

Michael J. Baine, Kavita Mallya, Surinder K. Batra

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Scopus citations

Abstract

The ability of peripheral blood mononuclear cells (PBMCs) to act as a surrogate window into the presence and physiologic effects of pancreatic cancer is becoming increasingly apparent. In this chapter, we describe the techniques for isolation, lysis, RNA extraction, cDNA synthesis, and Q-RT PCR analysis of PBMCs as well as reasonable alternatives and the advantages and disadvantages of each. We further discuss the noteworthy considerations necessary for successful isolation and conversion of the high-quality PBMC RNA required to acquire interpretable and reproducible results for PBMC genetic expression analysis.

Original languageEnglish (US)
Title of host publicationPancreatic Cancer
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages157-173
Number of pages17
ISBN (Print)9781627032865
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume980
ISSN (Print)1064-3745

Keywords

  • Blood
  • Cancer immunity
  • Genetic expression
  • Pancreatic cancer
  • Peripheral blood mononuclear cells (PBMCs)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Baine, M. J., Mallya, K., & Batra, S. K. (2013). Quantitative real-time PCR expression analysis of peripheral blood mononuclear cells in pancreatic cancer patients. In Pancreatic Cancer: Methods and Protocols (pp. 157-173). (Methods in Molecular Biology; Vol. 980). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-287-2_8