TY - JOUR
T1 - Quorum sensing activity and control of yeast-mycelium dimorphism in Ophiostoma floccosum
AU - Berrocal, Alexander
AU - Oviedo, Claudia
AU - Nickerson, Kenneth W.
AU - Navarrete, José
N1 - Funding Information:
Acknowledgments The principal author would like to thank the personal of the Laboratory of Biodegradation in the Department of Wood Engineering at the University of Bio– Bio (Chile) for all support. To the Graduate School and Department of Wood Engineering at the University of Bio–Bio, the OEA Scholarship Program and the Scholarship Program of the Technology Institute of Costa Rica for providing the funds to carry out this research. We also thank Ph.D. Juanita Freer and Susana Casas from the Laboratory of Renewable Resources at the University of Concepcion for providing the facilities for GC–MS analyses.
PY - 2014/6
Y1 - 2014/6
N2 - Quorum sensing (QS) activity in Ophiostoma fungi has not been described. We have examined the growth conditions on the control of dimorphism in Ophiostoma floccosum, an attractive biocontrol agent against blue-stain fungi, and its relationship with QS activity. In a defined culture medium with l-proline as the N source, a high inoculum size (107 c.f.u. ml-1) was the principal factor that promoted yeast-like growth. Inoculum size effect can be explained by the secretion of a QS molecule(s) (QSMs) responsible for inducing yeast morphology. QSM candidates were extracted from spent medium and their structure was determined by GC-MS. Three cyclic sesquiterpenes were found. The most abundant molecule, and therefore the principal candidate to be the QSM responsible for yeast growth of O. floccosum, was 1,1,4a-trimethyl-5,6-dimethylene-decalin (C15H24). Other two compounds were also detected.
AB - Quorum sensing (QS) activity in Ophiostoma fungi has not been described. We have examined the growth conditions on the control of dimorphism in Ophiostoma floccosum, an attractive biocontrol agent against blue-stain fungi, and its relationship with QS activity. In a defined culture medium with l-proline as the N source, a high inoculum size (107 c.f.u. ml-1) was the principal factor that promoted yeast-like growth. Inoculum size effect can be explained by the secretion of a QS molecule(s) (QSMs) responsible for inducing yeast morphology. QSM candidates were extracted from spent medium and their structure was determined by GC-MS. Three cyclic sesquiterpenes were found. The most abundant molecule, and therefore the principal candidate to be the QSM responsible for yeast growth of O. floccosum, was 1,1,4a-trimethyl-5,6-dimethylene-decalin (C15H24). Other two compounds were also detected.
KW - Cyclic sesquiterpenes
KW - Fungal dimorphism
KW - Inoculum size
KW - Ophiostoma floccosum
KW - Quorum sensing molecules
KW - Trimethyl-dimethylene-decalin
UR - http://www.scopus.com/inward/record.url?scp=84902244823&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84902244823&partnerID=8YFLogxK
U2 - 10.1007/s10529-014-1514-5
DO - 10.1007/s10529-014-1514-5
M3 - Article
C2 - 24737073
AN - SCOPUS:84902244823
SN - 0141-5492
VL - 36
SP - 1503
EP - 1513
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 7
ER -