Radioligand-binding methods for membrane preparations and intact cells.

David B. Bylund, Jean D. Deupree, Myron L. Toews

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The radioligand-binding assay is a relatively simple but powerful tool for studying G-protein-coupled receptors. There are three basic types of radioligand-binding experiments: (1) saturation experiments from which the affinity of the radioligand for the receptor and the binding site density can be determined; (2) inhibition experiments from which the affinity of a competing, unlabeled compound for the receptor can be determined: and (3) kinetic experiments from which the forward and reverse rate constants for radioligand binding can be determined. Detailed methods for typical radioligand-binding assays for G-protein-coupled receptors in membranes and intact cells are presented for these types of experiments. Detailed procedures for analysis of the data obtained from these experiments are also given.

Original languageEnglish (US)
Pages (from-to)1-28
Number of pages28
JournalMethods in molecular biology (Clifton, N.J.)
Volume259
StatePublished - 2004

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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