Ranque-hilsch vortex tube thermocycler for DNA amplification

R. Ebmeier, S. Whitney, S. Alugupally, M. Nelson, N. Padhye, G. Gogos, H. J. Viljoen

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

An innovative polymerase chain reaction (PCR) thermocycler using pressurized gas through a Ranque-Hilsch vortex tube is described below. This device can amplify 10pg of 186bp Escherichia coli uidA amplicon in a 20 μL sample 3.3 × 108-fold, by performing 35 cycles in less than 8 min. This PCR amplification corresponds to an overall efficiency of 75%.

Original languageEnglish (US)
Pages (from-to)567-570
Number of pages4
JournalInstrumentation Science and Technology
Volume32
Issue number5
DOIs
StatePublished - Sep 1 2004

Keywords

  • DNA amplification
  • PCR method
  • Reaction time
  • Thermocycles

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Instrumentation
  • Environmental Science(all)

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