Abstract
The use of Ranque-Hilsch Vortex Tube Thermocycler for 96 bp λ-DNA amplicon and real-time optical detection was discussed. The speed of the Ranque-Hilsch Vortex Tube Thermocycler was best utilized in combination with a fast enzyme such as KOD Hot Start Polymerase. It was found that the total reaction time which includes the 30 second hot start and subsequent 45 cycles between the denaturation, annealing and elongation temperatures, was less than 12 minutes. The results show that the 0.25 fg DNA sample surpassed the threshold value 8 minutes into the reaction at cycle number 34.
Original language | English (US) |
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Title of host publication | AIChE Annual Meeting, Conference Proceedings |
Pages | 10479-10485 |
Number of pages | 7 |
State | Published - 2004 |
Event | 2004 AIChE Annual Meeting - Austin, TX Duration: Nov 7 2004 → Nov 12 2004 |
Other
Other | 2004 AIChE Annual Meeting |
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City | Austin, TX |
Period | 11/7/04 → 11/12/04 |
ASJC Scopus subject areas
- Engineering(all)