Ranque-Hilsch vortex tube thermocycler for fast DNA amplification and real-time optical detection

Ryan J. Ebmeier; George Gogos; Scott E. Whitney; Amitabha Sarkar; Hendrik J. Viljoen; Michael Nelson; Nisha V. Padhye

Ranque-Hilsch vortex tube thermocycler for fast DNA amplification and real-time optical detection

Ryan J. Ebmeier, George Gogos, Scott E. Whitney, Amitabha Sarkar, Hendrik J. Viljoen, Michael Nelson, Nisha V. Padhye

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The use of Ranque-Hilsch Vortex Tube Thermocycler for 96 bp λ-DNA amplicon and real-time optical detection was discussed. The speed of the Ranque-Hilsch Vortex Tube Thermocycler was best utilized in combination with a fast enzyme such as KOD Hot Start Polymerase. It was found that the total reaction time which includes the 30 second hot start and subsequent 45 cycles between the denaturation, annealing and elongation temperatures, was less than 12 minutes. The results show that the 0.25 fg DNA sample surpassed the threshold value 8 minutes into the reaction at cycle number 34.

Original language	English (US)
Title of host publication	AIChE Annual Meeting, Conference Proceedings
Pages	10479-10485
Number of pages	7
State	Published - 2004
Event	2004 AIChE Annual Meeting - Austin, TX Duration: Nov 7 2004 → Nov 12 2004

Other

Other	2004 AIChE Annual Meeting
City	Austin, TX
Period	11/7/04 → 11/12/04

ASJC Scopus subject areas

General Engineering

Cite this

@inproceedings{7f91751d1794403a9bc84b082a697131,

title = "Ranque-Hilsch vortex tube thermocycler for fast DNA amplification and real-time optical detection",

abstract = "The use of Ranque-Hilsch Vortex Tube Thermocycler for 96 bp λ-DNA amplicon and real-time optical detection was discussed. The speed of the Ranque-Hilsch Vortex Tube Thermocycler was best utilized in combination with a fast enzyme such as KOD Hot Start Polymerase. It was found that the total reaction time which includes the 30 second hot start and subsequent 45 cycles between the denaturation, annealing and elongation temperatures, was less than 12 minutes. The results show that the 0.25 fg DNA sample surpassed the threshold value 8 minutes into the reaction at cycle number 34.",

author = "Ebmeier, {Ryan J.} and George Gogos and Whitney, {Scott E.} and Amitabha Sarkar and Viljoen, {Hendrik J.} and Michael Nelson and Padhye, {Nisha V.}",

year = "2004",

language = "English (US)",

pages = "10479--10485",

booktitle = "AIChE Annual Meeting, Conference Proceedings",

note = "2004 AIChE Annual Meeting ; Conference date: 07-11-2004 Through 12-11-2004",

}

TY - GEN

T1 - Ranque-Hilsch vortex tube thermocycler for fast DNA amplification and real-time optical detection

AU - Ebmeier, Ryan J.

AU - Gogos, George

AU - Whitney, Scott E.

AU - Sarkar, Amitabha

AU - Viljoen, Hendrik J.

AU - Nelson, Michael

AU - Padhye, Nisha V.

PY - 2004

Y1 - 2004

N2 - The use of Ranque-Hilsch Vortex Tube Thermocycler for 96 bp λ-DNA amplicon and real-time optical detection was discussed. The speed of the Ranque-Hilsch Vortex Tube Thermocycler was best utilized in combination with a fast enzyme such as KOD Hot Start Polymerase. It was found that the total reaction time which includes the 30 second hot start and subsequent 45 cycles between the denaturation, annealing and elongation temperatures, was less than 12 minutes. The results show that the 0.25 fg DNA sample surpassed the threshold value 8 minutes into the reaction at cycle number 34.

AB - The use of Ranque-Hilsch Vortex Tube Thermocycler for 96 bp λ-DNA amplicon and real-time optical detection was discussed. The speed of the Ranque-Hilsch Vortex Tube Thermocycler was best utilized in combination with a fast enzyme such as KOD Hot Start Polymerase. It was found that the total reaction time which includes the 30 second hot start and subsequent 45 cycles between the denaturation, annealing and elongation temperatures, was less than 12 minutes. The results show that the 0.25 fg DNA sample surpassed the threshold value 8 minutes into the reaction at cycle number 34.

UR - http://www.scopus.com/inward/record.url?scp=22544469470&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=22544469470&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:22544469470

SP - 10479

EP - 10485

BT - AIChE Annual Meeting, Conference Proceedings

T2 - 2004 AIChE Annual Meeting

Y2 - 7 November 2004 through 12 November 2004

ER -

Ranque-Hilsch vortex tube thermocycler for fast DNA amplification and real-time optical detection

Abstract

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ASJC Scopus subject areas

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