TY - JOUR
T1 - Rapid and reliable quantitation of amino acids and myo-inositol in mouse brain by high performance liquid chromatography and tandem mass spectrometry
AU - Bathena, Sai P.
AU - Huang, Jiangeng
AU - Epstein, Adrian A.
AU - Gendelman, Howard E.
AU - Boska, Michael D.
AU - Alnouti, Yazen
N1 - Funding Information:
This work was supported in part by NIH grant PO1 DA028555-01 .
PY - 2012/4/15
Y1 - 2012/4/15
N2 - Amino acids and myo-inositol have long been proposed as putative biomarkers for neurodegenerative diseases. Accurate measures and stability have precluded their selective use. To this end, a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method based on multiple reaction monitoring was developed to simultaneously quantify glutamine, glutamate, γ-aminobutyric acid (GABA), aspartic acid, N-acetyl aspartic acid, taurine, choline, creatine, phosphocholine and myo-inositol in mouse brain by methanol extractions. Chromatography was performed using a hydrophilic interaction chromatography silica column within in a total run time of 15. min. The validated method is selective, sensitive, accurate, and precise. The method has a limit of quantification ranging from 2.5 to 20. ng/ml for a range of analytes and a dynamic range from 2.5-20 to 500-4000. ng/ml. This LC-MS/MS method was validated for biomarker discovery in models of human neurological disorders.
AB - Amino acids and myo-inositol have long been proposed as putative biomarkers for neurodegenerative diseases. Accurate measures and stability have precluded their selective use. To this end, a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method based on multiple reaction monitoring was developed to simultaneously quantify glutamine, glutamate, γ-aminobutyric acid (GABA), aspartic acid, N-acetyl aspartic acid, taurine, choline, creatine, phosphocholine and myo-inositol in mouse brain by methanol extractions. Chromatography was performed using a hydrophilic interaction chromatography silica column within in a total run time of 15. min. The validated method is selective, sensitive, accurate, and precise. The method has a limit of quantification ranging from 2.5 to 20. ng/ml for a range of analytes and a dynamic range from 2.5-20 to 500-4000. ng/ml. This LC-MS/MS method was validated for biomarker discovery in models of human neurological disorders.
KW - Amino acids
KW - HILIC
KW - LC-MS/MS
KW - Multiple reaction monitoring (MRM)
KW - Myo-inositol
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U2 - 10.1016/j.jchromb.2012.01.035
DO - 10.1016/j.jchromb.2012.01.035
M3 - Article
C2 - 22425384
AN - SCOPUS:84862790001
SN - 1570-0232
VL - 893-894
SP - 15
EP - 20
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ER -