Rapid and specific detection of Asian- and African-lineage Zika viruses

Nunya Chotiwan; Connie D. Brewster; Tereza Magalhaes; James Weger-Lucarelli; Nisha K. Duggal; Claudia Rückert; Chilinh Nguyen; Selene M.Garcia Luna; Joseph R. Fauver; Barb Andre; Meg Gray; William C.Black Iv; Rebekah C. Kading; Gregory D. Ebel; Guillermina Kuan; Angel Balmaseda; Thomas Jaenisch; Ernesto T.A. Marques; Aaron C. Brault; Eva Harris; Brian D. Foy; Sandra L. Quackenbush; Rushika Perera; Joel Rovnak

doi:10.1126/scitranslmed.aag0538

Rapid and specific detection of Asian- and African-lineage Zika viruses

Nunya Chotiwan, Connie D. Brewster, Tereza Magalhaes, James Weger-Lucarelli, Nisha K. Duggal, Claudia Rückert, Chilinh Nguyen, Selene M.Garcia Luna, Joseph R. Fauver, Barb Andre, Meg Gray, William C.Black Iv, Rebekah C. Kading, Gregory D. Ebel, Guillermina Kuan, Angel Balmaseda, Thomas Jaenisch, Ernesto T.A. Marques, Aaron C. Brault, Eva HarrisBrian D. Foy, Sandra L. Quackenbush, Rushika Perera, Joel Rovnak

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84 Scopus citations

Abstract

Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers.

Original language	English (US)
Article number	aag0538
Journal	Science translational medicine
Volume	9
Issue number	388
DOIs	https://doi.org/10.1126/scitranslmed.aag0538
State	Published - May 3 2017
Externally published	Yes

ASJC Scopus subject areas

General Medicine

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Chotiwan, N., Brewster, C. D., Magalhaes, T., Weger-Lucarelli, J., Duggal, N. K., Rückert, C., Nguyen, C., Luna, S. M. G., Fauver, J. R., Andre, B., Gray, M., Iv, W. C. B., Kading, R. C., Ebel, G. D., Kuan, G., Balmaseda, A., Jaenisch, T., Marques, E. T. A., Brault, A. C., ... Rovnak, J. (2017). Rapid and specific detection of Asian- and African-lineage Zika viruses. Science translational medicine, 9(388), Article aag0538. https://doi.org/10.1126/scitranslmed.aag0538

Chotiwan, N, Brewster, CD, Magalhaes, T, Weger-Lucarelli, J, Duggal, NK, Rückert, C, Nguyen, C, Luna, SMG, Fauver, JR, Andre, B, Gray, M, Iv, WCB, Kading, RC, Ebel, GD, Kuan, G, Balmaseda, A, Jaenisch, T, Marques, ETA, Brault, AC, Harris, E, Foy, BD, Quackenbush, SL, Perera, R & Rovnak, J 2017, 'Rapid and specific detection of Asian- and African-lineage Zika viruses', Science translational medicine, vol. 9, no. 388, aag0538. https://doi.org/10.1126/scitranslmed.aag0538

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abstract = "Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers.",

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AU - Jaenisch, Thomas

AU - Marques, Ernesto T.A.

AU - Brault, Aaron C.

AU - Harris, Eva

AU - Foy, Brian D.

AU - Quackenbush, Sandra L.

AU - Perera, Rushika

AU - Rovnak, Joel

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Rapid and specific detection of Asian- and African-lineage Zika viruses

Abstract

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