Rapid chromatographic purification of apolipoproteins A-I and A-II from human plasma

Sherman E. Ross; Steven D. Carson

doi:10.1016/0003-2697(85)90490-7

Rapid chromatographic purification of apolipoproteins A-I and A-II from human plasma

Sherman E. Ross, Steven D. Carson

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Rapid, large-scale isolation of human apolipoproteins A-I and A-II has been accomplished using two chromatographic procedures. The apolipoproteins adsorbed from plasma onto a column of phenyl-Sepharose are eluted with increasing propylene glycol concentrations. Apolipoproteins A-I and A-II can be resolved by elution with a linear 0 to 80% propylene glycol gradient. Homogeneous preparations of apo A-I and A-II are obtained following gel filtration in 3 m guanidinium chloride.

Original language	English (US)
Pages (from-to)	166-168
Number of pages	3
Journal	Analytical Biochemistry
Volume	149
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(85)90490-7
State	Published - Aug 15 1985
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(85)90490-7

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title = "Rapid chromatographic purification of apolipoproteins A-I and A-II from human plasma",

abstract = "Rapid, large-scale isolation of human apolipoproteins A-I and A-II has been accomplished using two chromatographic procedures. The apolipoproteins adsorbed from plasma onto a column of phenyl-Sepharose are eluted with increasing propylene glycol concentrations. Apolipoproteins A-I and A-II can be resolved by elution with a linear 0 to 80% propylene glycol gradient. Homogeneous preparations of apo A-I and A-II are obtained following gel filtration in 3 m guanidinium chloride.",

author = "Ross, {Sherman E.} and Carson, {Steven D.}",

note = "Funding Information: We gratefully acknowledge Mr. Howard Mitchell for preparation of the figures. This work was supported by Grant HL-3 1408 from the National Institutes of Health, and by BRSG-05357 awarded by the Biomedical Research Grant Program, Division of Research Resources National Institutes of Health. A departmental gift from R. J. Reynolds Industries, Inc. and Grant CA-15823 from the National Institutes of Health provided departmental facilities.",

year = "1985",

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doi = "10.1016/0003-2697(85)90490-7",

language = "English (US)",

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pages = "166--168",

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T1 - Rapid chromatographic purification of apolipoproteins A-I and A-II from human plasma

AU - Ross, Sherman E.

AU - Carson, Steven D.

N1 - Funding Information: We gratefully acknowledge Mr. Howard Mitchell for preparation of the figures. This work was supported by Grant HL-3 1408 from the National Institutes of Health, and by BRSG-05357 awarded by the Biomedical Research Grant Program, Division of Research Resources National Institutes of Health. A departmental gift from R. J. Reynolds Industries, Inc. and Grant CA-15823 from the National Institutes of Health provided departmental facilities.

PY - 1985/8/15

Y1 - 1985/8/15

N2 - Rapid, large-scale isolation of human apolipoproteins A-I and A-II has been accomplished using two chromatographic procedures. The apolipoproteins adsorbed from plasma onto a column of phenyl-Sepharose are eluted with increasing propylene glycol concentrations. Apolipoproteins A-I and A-II can be resolved by elution with a linear 0 to 80% propylene glycol gradient. Homogeneous preparations of apo A-I and A-II are obtained following gel filtration in 3 m guanidinium chloride.

AB - Rapid, large-scale isolation of human apolipoproteins A-I and A-II has been accomplished using two chromatographic procedures. The apolipoproteins adsorbed from plasma onto a column of phenyl-Sepharose are eluted with increasing propylene glycol concentrations. Apolipoproteins A-I and A-II can be resolved by elution with a linear 0 to 80% propylene glycol gradient. Homogeneous preparations of apo A-I and A-II are obtained following gel filtration in 3 m guanidinium chloride.

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JO - Analytical Biochemistry

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Rapid chromatographic purification of apolipoproteins A-I and A-II from human plasma

Abstract

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