Rapid deposition of wheat cell wall structural proteins in response to Fusarium-derived elicitors

W. El-Gendy; M. D. Brownleader; H. Ismail; P. J. Clarke; J. Gilbert; F. El-Bordiny; M. Trevan; J. Hopkins; M. Naldrett; P. Jackson

doi:10.1093/jxb/52.354.85

Rapid deposition of wheat cell wall structural proteins in response to Fusarium-derived elicitors

W. El-Gendy, M. D. Brownleader, H. Ismail, P. J. Clarke, J. Gilbert, F. El-Bordiny, M. Trevan, J. Hopkins, M. Naldrett, P. Jackson

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Two novel cell wall structural proteins of spring wheat (Triticum aestivum L. em Thell.), undergo rapid deposition in the cell wall matrix in a H₂O₂-dependent reaction after the elicitation of cultures with Fusarium graminearum (L.)-derived elicitor. The amino acid compositions of these proteins were remarkably similar and indicated that they were highly acidic (pl 3.8). These proteins contained 13-17% each of Gly, Glx and Ser with lesser amounts (6-8%) of Ala, Asx and Thr, and it has been suggested that they are known as glycine- and serine-rich proteins (GSRPs). SELDI-MS ionization spectra demonstrated that these proteins have low molecular masses of 8 590 and 4 292 Da. These results are discussed in relation to the possible role of these novel proteins in rapid, cell wall defensive reactions to pathogenic attack.

Original language	English (US)
Pages (from-to)	85-90
Number of pages	6
Journal	Journal of experimental botany
Volume	52
Issue number	354
DOIs	https://doi.org/10.1093/jxb/52.354.85
State	Published - 2001
Externally published	Yes

Keywords

Cell wall
Elicitation
Structural proteins
Triticum aestivum L.

ASJC Scopus subject areas

Physiology
Plant Science

Access to Document

10.1093/jxb/52.354.85

Cite this

@article{ecf0703738244a338f3c2459f666a99a,

title = "Rapid deposition of wheat cell wall structural proteins in response to Fusarium-derived elicitors",

abstract = "Two novel cell wall structural proteins of spring wheat (Triticum aestivum L. em Thell.), undergo rapid deposition in the cell wall matrix in a H2O2-dependent reaction after the elicitation of cultures with Fusarium graminearum (L.)-derived elicitor. The amino acid compositions of these proteins were remarkably similar and indicated that they were highly acidic (pl 3.8). These proteins contained 13-17% each of Gly, Glx and Ser with lesser amounts (6-8%) of Ala, Asx and Thr, and it has been suggested that they are known as glycine- and serine-rich proteins (GSRPs). SELDI-MS ionization spectra demonstrated that these proteins have low molecular masses of 8 590 and 4 292 Da. These results are discussed in relation to the possible role of these novel proteins in rapid, cell wall defensive reactions to pathogenic attack.",

keywords = "Cell wall, Elicitation, Structural proteins, Triticum aestivum L.",

author = "W. El-Gendy and Brownleader, {M. D.} and H. Ismail and Clarke, {P. J.} and J. Gilbert and F. El-Bordiny and M. Trevan and J. Hopkins and M. Naldrett and P. Jackson",

note = "Funding Information: Wael El-Gendy is grateful to The British Council Chevening Section for financial support. Dr Philip Jackson acknowledges a research fellowship awarded by PRAXIS XXI (ref. BPDu 16316u98). We thank Dr Lee Lomas (Ciphergen, UK) for performing SELDI-mass spectrometric analyses.",

year = "2001",

doi = "10.1093/jxb/52.354.85",

language = "English (US)",

volume = "52",

pages = "85--90",

journal = "Journal of Experimental Botany",

issn = "0022-0957",

publisher = "Oxford University Press",

number = "354",

}

TY - JOUR

T1 - Rapid deposition of wheat cell wall structural proteins in response to Fusarium-derived elicitors

AU - El-Gendy, W.

AU - Brownleader, M. D.

AU - Ismail, H.

AU - Clarke, P. J.

AU - Gilbert, J.

AU - El-Bordiny, F.

AU - Trevan, M.

AU - Hopkins, J.

AU - Naldrett, M.

AU - Jackson, P.

N1 - Funding Information: Wael El-Gendy is grateful to The British Council Chevening Section for financial support. Dr Philip Jackson acknowledges a research fellowship awarded by PRAXIS XXI (ref. BPDu 16316u98). We thank Dr Lee Lomas (Ciphergen, UK) for performing SELDI-mass spectrometric analyses.

PY - 2001

Y1 - 2001

N2 - Two novel cell wall structural proteins of spring wheat (Triticum aestivum L. em Thell.), undergo rapid deposition in the cell wall matrix in a H2O2-dependent reaction after the elicitation of cultures with Fusarium graminearum (L.)-derived elicitor. The amino acid compositions of these proteins were remarkably similar and indicated that they were highly acidic (pl 3.8). These proteins contained 13-17% each of Gly, Glx and Ser with lesser amounts (6-8%) of Ala, Asx and Thr, and it has been suggested that they are known as glycine- and serine-rich proteins (GSRPs). SELDI-MS ionization spectra demonstrated that these proteins have low molecular masses of 8 590 and 4 292 Da. These results are discussed in relation to the possible role of these novel proteins in rapid, cell wall defensive reactions to pathogenic attack.

AB - Two novel cell wall structural proteins of spring wheat (Triticum aestivum L. em Thell.), undergo rapid deposition in the cell wall matrix in a H2O2-dependent reaction after the elicitation of cultures with Fusarium graminearum (L.)-derived elicitor. The amino acid compositions of these proteins were remarkably similar and indicated that they were highly acidic (pl 3.8). These proteins contained 13-17% each of Gly, Glx and Ser with lesser amounts (6-8%) of Ala, Asx and Thr, and it has been suggested that they are known as glycine- and serine-rich proteins (GSRPs). SELDI-MS ionization spectra demonstrated that these proteins have low molecular masses of 8 590 and 4 292 Da. These results are discussed in relation to the possible role of these novel proteins in rapid, cell wall defensive reactions to pathogenic attack.

KW - Cell wall

KW - Elicitation

KW - Structural proteins

KW - Triticum aestivum L.

UR - http://www.scopus.com/inward/record.url?scp=0035096946&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035096946&partnerID=8YFLogxK

U2 - 10.1093/jxb/52.354.85

DO - 10.1093/jxb/52.354.85

M3 - Article

C2 - 11181716

AN - SCOPUS:0035096946

SN - 0022-0957

VL - 52

SP - 85

EP - 90

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

IS - 354

ER -

Rapid deposition of wheat cell wall structural proteins in response to Fusarium-derived elicitors

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this