Rapid determination of advanced glycation end products of proteins using MALDI-TOF-MS and PERL script peptide searching algorithm

Yinong Zhang, Ross R. Cocklin, Keshore R. Bidasee, Mu Wang

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Advanced glycation end products (AGEs), which are composed of various glucose or carbohydrate adducts, are thought to be responsible for several diabetic and age-related complications. However, to date, specific sites on proteins that are modified by AGEs remain largely unknown. We report here the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to determine the type and localization of several AGEs formed in vitro on human beta-2-microglobulin (β 2M),and in vivo on type 2 ryanodine receptor calcium-release channel (RyR2), and sarco(endo)plasmic reticulum (SERCA2a). A PERL script algorithm, developed in-house, makes searching the relatively large amount of data generated by the MALDI-MS more manageable.The outstanding sensitivity of MALDI-TOF-MS coupled with the PERL script algorithm allows such an approach to be a very useful tool in detecting AGEs and other post-translational modifications. We believe that this method could be an important tool when searching for post-translational modifications on proteins.

Original languageEnglish (US)
Pages (from-to)224-230
Number of pages7
JournalJournal of Biomolecular Techniques
Volume14
Issue number3
StatePublished - Sep 2003

Keywords

  • Advanced-glycation end products
  • Mass spectrometry
  • Matrix-assisted laser desorption/ionization
  • Post-translational modification

ASJC Scopus subject areas

  • Molecular Biology

Fingerprint Dive into the research topics of 'Rapid determination of advanced glycation end products of proteins using MALDI-TOF-MS and PERL script peptide searching algorithm'. Together they form a unique fingerprint.

Cite this