TY - JOUR
T1 - Rapid Image Reconstruction of Structured Illumination Microscopy Directly in the Spatial Domain
AU - Dan, Dan
AU - Wang, Zhaojun
AU - Zhou, Xing
AU - Lei, Ming
AU - Zhao, Tianyu
AU - Qian, Jia
AU - Yu, Xianghua
AU - Yan, Shaohui
AU - Min, Junwei
AU - Bianco, Piero R.
AU - Yao, Baoli
N1 - Publisher Copyright:
© 2009-2012 IEEE.
PY - 2021/2
Y1 - 2021/2
N2 - Super-resolution structured illumination microscopy (SIM) routinely performs image reconstruction in the frequency domain using an approach termed frequency-domain reconstruction (FDR). Due to multiple Fourier transforms between the spatial and frequency domains, SIM suffers from low reconstruction speed, constraining its applications in real-time, dynamic imaging. To overcome this limitation, we developed a new method for SIM image reconstruction, termed spatial domain reconstruction (SDR). SDR is intrinsically simpler than FDR, does not require Fourier transforms and the theory predicts it to be a rapid image reconstruction method. Results show that SDR reconstructs a super-resolution image 7-fold faster than FDR, producing images that are equal to either FDR or the widely-used FairSIM. We provide a proof-of-principle using mobile fluorescent beads to demonstrate the utility of SDR in imaging moving objects. Consequently, replacement of the FDR approach with SDR significantly enhances SIM as the desired method for live-cell, instant super-resolution imaging. This means that SDR-SIM is a 'What You See Is What You Get' approach to super-resolution imaging.
AB - Super-resolution structured illumination microscopy (SIM) routinely performs image reconstruction in the frequency domain using an approach termed frequency-domain reconstruction (FDR). Due to multiple Fourier transforms between the spatial and frequency domains, SIM suffers from low reconstruction speed, constraining its applications in real-time, dynamic imaging. To overcome this limitation, we developed a new method for SIM image reconstruction, termed spatial domain reconstruction (SDR). SDR is intrinsically simpler than FDR, does not require Fourier transforms and the theory predicts it to be a rapid image reconstruction method. Results show that SDR reconstructs a super-resolution image 7-fold faster than FDR, producing images that are equal to either FDR or the widely-used FairSIM. We provide a proof-of-principle using mobile fluorescent beads to demonstrate the utility of SDR in imaging moving objects. Consequently, replacement of the FDR approach with SDR significantly enhances SIM as the desired method for live-cell, instant super-resolution imaging. This means that SDR-SIM is a 'What You See Is What You Get' approach to super-resolution imaging.
KW - Super-resolution microscopy
KW - instant super-resolution imaging
KW - spatial domain reconstruction
KW - structured illumination
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U2 - 10.1109/JPHOT.2021.3053110
DO - 10.1109/JPHOT.2021.3053110
M3 - Article
C2 - 33880138
AN - SCOPUS:85099733041
SN - 1943-0655
VL - 13
JO - IEEE Photonics Journal
JF - IEEE Photonics Journal
IS - 1
M1 - 9329035
ER -