Rapid Image Reconstruction of Structured Illumination Microscopy Directly in the Spatial Domain

Dan Dan, Zhaojun Wang, Xing Zhou, Ming Lei, Tianyu Zhao, Jia Qian, Xianghua Yu, Shaohui Yan, Junwei Min, Piero R. Bianco, Baoli Yao

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Super-resolution structured illumination microscopy (SIM) routinely performs image reconstruction in the frequency domain using an approach termed frequency-domain reconstruction (FDR). Due to multiple Fourier transforms between the spatial and frequency domains, SIM suffers from low reconstruction speed, constraining its applications in real-time, dynamic imaging. To overcome this limitation, we developed a new method for SIM image reconstruction, termed spatial domain reconstruction (SDR). SDR is intrinsically simpler than FDR, does not require Fourier transforms and the theory predicts it to be a rapid image reconstruction method. Results show that SDR reconstructs a super-resolution image 7-fold faster than FDR, producing images that are equal to either FDR or the widely-used FairSIM. We provide a proof-of-principle using mobile fluorescent beads to demonstrate the utility of SDR in imaging moving objects. Consequently, replacement of the FDR approach with SDR significantly enhances SIM as the desired method for live-cell, instant super-resolution imaging. This means that SDR-SIM is a 'What You See Is What You Get' approach to super-resolution imaging.

Original languageEnglish (US)
Article number9329035
JournalIEEE Photonics Journal
Volume13
Issue number1
DOIs
StatePublished - Feb 2021

Keywords

  • Super-resolution microscopy
  • instant super-resolution imaging
  • spatial domain reconstruction
  • structured illumination

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering

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