Rapid purification of human placental aldose reductase

Peter F. Kador, Deborah Carper, Jin H. Kinoshita

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Aldose reductase (alditol:NADP oxidoreductase EC 1.1.1.21) an enzyme in the polyol pathway which has been implicated in the pathogenesis of diabetic complications has been purified from the human placenta. Using affinity chromatography the enzyme can be obtained with apparent homogeneity in three steps. An initial 30-70% ammonium sulfate fraction was placed on a 4-carboxybenzaldehyde-coupled AH-Sepharose 4B column where upon elution with 0.1 m Na,K-phosphate buffer, pH 6.2, an enzyme fraction separated from the major protein peak. Chromatography of this enzyme fraction on an Amicon Matrex Orange A dyeligand column and elution with phosphate buffer containing 0.1 mm NADPH yielded aldose reductase of high purity. Antibodies raised in rabbits against the purified enzyme gave an apparent single line of identity with cither crude or purified aldose reductase.

Original languageEnglish (US)
Pages (from-to)53-58
Number of pages6
JournalAnalytical Biochemistry
Volume114
Issue number1
DOIs
StatePublished - Jun 1981

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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