Rat embryo development in vitro

S. K. Roy; J. Sengupta; S. K. Manchanda

doi:10.1530/acta.0.0960546

Rat embryo development in vitro

S. K. Roy, J. Sengupta, S. K. Manchanda

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

We report the first successful culture of 8-cell/morula stage rat embryos in a fully synthetic medium supplemented with 3% crystalline bovine serum albumin. Eighty-four per cent of morulae developed to blastocysts, showing that this is a highly efficient culture medium for in vitro studies on rat pre-implantation embryos. Blastocyst formation was severely inhibited by antioestrogen (nafoxidine 3 μg/ml) but no further reversal was obtained by giving oestrogen to culture medium containing this antagonist.

Original language	English (US)
Pages (from-to)	546-551
Number of pages	6
Journal	Acta Endocrinologica
Volume	96
Issue number	4
DOIs	https://doi.org/10.1530/acta.0.0960546
State	Published - 1981
Externally published	Yes

ASJC Scopus subject areas

Endocrinology

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AU - Sengupta, J.

AU - Manchanda, S. K.

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AB - We report the first successful culture of 8-cell/morula stage rat embryos in a fully synthetic medium supplemented with 3% crystalline bovine serum albumin. Eighty-four per cent of morulae developed to blastocysts, showing that this is a highly efficient culture medium for in vitro studies on rat pre-implantation embryos. Blastocyst formation was severely inhibited by antioestrogen (nafoxidine 3 μg/ml) but no further reversal was obtained by giving oestrogen to culture medium containing this antagonist.

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Rat embryo development in vitro

Abstract

ASJC Scopus subject areas

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