TY - JOUR
T1 - Rat gastric gelatinase induction during endotoxemia
AU - Robinson, Emily K.
AU - West, Sonlee D.
AU - Garay, Alian
AU - Mercer, David W.
N1 - Funding Information:
This work was supported by NIGMS Grants GM-070812 (E.K.R.), GM-08792 (D.W.M.), and GM-38529 (D.W.M.) and by NIH Grant DK56338, which supports the Texas Gulf Coast Digestive Diseases Center (E.K.R.).
PY - 2006/3
Y1 - 2006/3
N2 - Despite continued investigation, the pathogenesis of tissue injury secondary to sepsis remains elusive. Further evaluation of the mechanisms by which endotoxemia and sepsis induce tissue injury is necessary to formulate rational and effective treatment strategies. The purpose of these studies was to evaluate the role of the matrix metalloproteinases MMP-2 and MMP-9 in gastric injury during lipopolysaccharide induced endotoxemia. Lipopolysaccharide increased gastric gelatinase activity as determined by in situ and gelatin zymography. Specifically, lipopolysaccharide induced MMP-2, MMP-9, and tissue inhibitor of metalloproteinase-1 (TIMP-1) transcription, with subsequent increases in MMP-2 and TIMP-1 protein expression. Furthermore, selective metalloproteinase inhibition ameliorated gastric injury in this model. These data suggest that lipopolysaccharide-induced gastric injury is mediated, at least in part, by increased MMP-2 production.
AB - Despite continued investigation, the pathogenesis of tissue injury secondary to sepsis remains elusive. Further evaluation of the mechanisms by which endotoxemia and sepsis induce tissue injury is necessary to formulate rational and effective treatment strategies. The purpose of these studies was to evaluate the role of the matrix metalloproteinases MMP-2 and MMP-9 in gastric injury during lipopolysaccharide induced endotoxemia. Lipopolysaccharide increased gastric gelatinase activity as determined by in situ and gelatin zymography. Specifically, lipopolysaccharide induced MMP-2, MMP-9, and tissue inhibitor of metalloproteinase-1 (TIMP-1) transcription, with subsequent increases in MMP-2 and TIMP-1 protein expression. Furthermore, selective metalloproteinase inhibition ameliorated gastric injury in this model. These data suggest that lipopolysaccharide-induced gastric injury is mediated, at least in part, by increased MMP-2 production.
KW - Gelatinase
KW - Lipopolysaccharide
KW - Matrix metalloproteinase
KW - Tissue inhibitor of metalloproteinase (TIMP)
KW - Zymography
UR - http://www.scopus.com/inward/record.url?scp=33645856775&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33645856775&partnerID=8YFLogxK
U2 - 10.1007/s10620-006-3169-0
DO - 10.1007/s10620-006-3169-0
M3 - Article
C2 - 16614966
AN - SCOPUS:33645856775
SN - 0163-2116
VL - 51
SP - 548
EP - 559
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
IS - 3
ER -