Real-time dynamic imaging of virus distribution In Vivo

Sean E. Hofherr, Kristen E. Adams, Christopher Y. Chen, Shannon May, Eric A. Weaver, Michael A. Barry

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The distribution of viruses and gene therapy vectors is difficult to assess in a living organism. For instance, trafficking in murine models can usually only be assessed after sacrificing the animal for tissue sectioning or extraction. These assays are laborious requiring whole animal sectioning to ascertain tissue localization. They also obviate the ability to perform longitudinal or kinetic studies in one animal. To track viruses after systemic infection, we have labeled adenoviruses with a near-infrared (NIR) fluorophore and imaged these after intravenous injection in mice. Imaging was able to track and quantitate virus particles entering the jugular vein simultaneous with injection, appearing in the heart within 500 milliseconds, distributing in the bloodstream and throughout the animal within 7 seconds, and that the bulk of virus distribution was essentially complete within 3 minutes. These data provide the first in vivo real-time tracking of the rapid initial events of systemic virus infection.

Original languageEnglish (US)
Article numbere17076
JournalPloS one
Volume6
Issue number2
DOIs
StatePublished - 2011

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Hofherr, S. E., Adams, K. E., Chen, C. Y., May, S., Weaver, E. A., & Barry, M. A. (2011). Real-time dynamic imaging of virus distribution In Vivo. PloS one, 6(2), [e17076]. https://doi.org/10.1371/journal.pone.0017076