Receptor-mediated internalization promotes increased endosome size and number in a RAB4- and RAB5-dependent manner

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Abstract

Despite their significance in receptor-mediated internalization and continued signal transduction in cells, early/sorting endosomes (EE/SE) remain incompletely characterized, with many outstanding questions that surround the dynamics of their size and number. While several studies have reported increases in EE/SE size and number resulting from endocytic events, few studies have addressed such dynamics in a methodological and quantitative manner. Herein we apply quantitative fluorescence microscopy to measure the size and number of EE/SE upon internalization of two different ligands: transferrin and epidermal growth factor. Additionally, we used siRNA knock-down to determine the involvement of 5 different endosomal RAB proteins (RAB4, RAB5, RAB8A, RAB10 and RAB11A) in EE/SE dynamics. Our study provides new information on the dynamics of endosomes during endocytosis, an important reference for researchers studying receptor-mediated internalization and endocytic events.

Original languageEnglish (US)
Article number151339
JournalEuropean Journal of Cell Biology
Volume102
Issue number3
DOIs
StatePublished - Sep 2023

Keywords

  • Endosome number
  • Endosome size
  • Internalization
  • RAB10
  • RAB11A
  • RAB4
  • RAB5
  • RAB8A

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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