Recombinant plasmid expressing the entire coding region of the Bmyc putative protein.

M. Steinitz, S. Ingvarsson, C. Asker, J. Sümegi, G. Klein

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

An expression vector with the entire coding region of Bmyc oncogene was constructed. The longest predicted open reading frame of Bmyc (178 amino acid residues) was expressed as a fusion protein with the trpE protein (308 amino acid residues) in transformed bacteria. The newly synthesized 58 Kd protein reacted with an anti pan-myc serum. The fusion protein isolated from a preparative Western blot was used as immunogen to generate rabbit anti myc specific immune sera.

Original languageEnglish (US)
Pages (from-to)49-54
Number of pages6
JournalJournal of clinical & laboratory immunology
Volume32
Issue number2
StatePublished - Jun 1990

ASJC Scopus subject areas

  • Immunology

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    Steinitz, M., Ingvarsson, S., Asker, C., Sümegi, J., & Klein, G. (1990). Recombinant plasmid expressing the entire coding region of the Bmyc putative protein. Journal of clinical & laboratory immunology, 32(2), 49-54.