Abstract
An expression vector with the entire coding region of Bmyc oncogene was constructed. The longest predicted open reading frame of Bmyc (178 amino acid residues) was expressed as a fusion protein with the trpE protein (308 amino acid residues) in transformed bacteria. The newly synthesized 58 Kd protein reacted with an anti pan-myc serum. The fusion protein isolated from a preparative Western blot was used as immunogen to generate rabbit anti myc specific immune sera.
Original language | English (US) |
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Pages (from-to) | 49-54 |
Number of pages | 6 |
Journal | Journal of clinical & laboratory immunology |
Volume | 32 |
Issue number | 2 |
State | Published - Jun 1990 |
Externally published | Yes |
ASJC Scopus subject areas
- Immunology