TY - JOUR
T1 - Reconstruction of Pathways Associated with Amino Acid Metabolism in Human Mitochondria
AU - Guda, Purnima
AU - Guda, Chittibabu
AU - Subramaniam, Shankar
N1 - Funding Information:
This work was supported by the UC Life Sciences Informatics Program and Mitokor Corporation ( L99-10077 ) to SS and the start-up funds to CG from the State University of New York at Albany.
PY - 2007/3
Y1 - 2007/3
N2 - We have used a bioinformatics approach for the identification and reconstruction of metabolic pathways associated with amino acid metabolism in human mitochondria. Human mitochondrial proteins determined by experimental and computational methods have been superposed on the reference pathways from the KEGG database to identify mitochondrial pathways. Enzymes at the entry and exit points for each reconstructed pathway were identified, and mitochondrial solute carrier proteins were determined where applicable. Intermediate enzymes in the mitochondrial pathways were identified based on the annotations available from public databases, evidence in current literature, or our MITOPRED program, which predicts the mitochondrial localization of proteins. Through integration of the data derived from experimental, bibliographical, and computational sources, we reconstructed the amino acid metabolic pathways in human mitochondria, which could help better understand the mitochondrial metabolism and its role in human health.
AB - We have used a bioinformatics approach for the identification and reconstruction of metabolic pathways associated with amino acid metabolism in human mitochondria. Human mitochondrial proteins determined by experimental and computational methods have been superposed on the reference pathways from the KEGG database to identify mitochondrial pathways. Enzymes at the entry and exit points for each reconstructed pathway were identified, and mitochondrial solute carrier proteins were determined where applicable. Intermediate enzymes in the mitochondrial pathways were identified based on the annotations available from public databases, evidence in current literature, or our MITOPRED program, which predicts the mitochondrial localization of proteins. Through integration of the data derived from experimental, bibliographical, and computational sources, we reconstructed the amino acid metabolic pathways in human mitochondria, which could help better understand the mitochondrial metabolism and its role in human health.
KW - amino acid metabolism
KW - human
KW - metabolic pathways
KW - mitochondria
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U2 - 10.1016/S1672-0229(08)60004-2
DO - 10.1016/S1672-0229(08)60004-2
M3 - Article
C2 - 18267298
AN - SCOPUS:38949119434
SN - 1672-0229
VL - 5
SP - 166
EP - 176
JO - Genomics, Proteomics and Bioinformatics
JF - Genomics, Proteomics and Bioinformatics
IS - 3-4
ER -