Recycling to the plasma membrane is delayed in EHD1 knockout mice

Debora Rapaport, Wojtek Auerbach, Naava Naslavsky, Metsada Pasmanik-Chor, Emilia Galperin, Amos Fein, Steve Caplan, Alexandra L. Joyner, Mia Horowitz

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

EHD1 is a member of the EHD family that contains four mammalian homologs. Among the invertebrate orthologs are a single Drosophila and Caenorhabditis elegans proteins and two plant members. They all contain three modules, a N-terminal domain that contains nucleotidebinding motifs, a central coiled-coil domain involved in oligomerization and a C-terminal region that harbors the EH domain. Studies in C. elegans and EHD1 depletion by RNA interference in human cells have demonstrated that it regulates recycling of membrane proteins. We addressed the physiological role of EHD1 through its inactivation in the mouse. Ehd1 knockout mice were indistinguishable from normal mice, had a normal life span and showed no histological abnormalities. Analysis of transferrin uptake in Ehd1-/- embryonic fibroblasts demonstrated delayed recycling to the plasma membrane with accumulation of transferrin in the endocytic recycling compartment. Our results corroborate theestablished role of EHD1 in the exit of membrane proteins from recycling endosomes in vivo in a mouse model.

Original languageEnglish (US)
Pages (from-to)52-60
Number of pages9
JournalTraffic
Volume7
Issue number1
DOIs
StatePublished - Jan 1 2006

Keywords

  • EH domain
  • EHD1
  • Endocytosis
  • Knockout technology

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Rapaport, D., Auerbach, W., Naslavsky, N., Pasmanik-Chor, M., Galperin, E., Fein, A., Caplan, S., Joyner, A. L., & Horowitz, M. (2006). Recycling to the plasma membrane is delayed in EHD1 knockout mice. Traffic, 7(1), 52-60. https://doi.org/10.1111/j.1600-0854.2005.00359.x