Redox-regulatory mechanisms induced by oxidative stress in Brassica juncea roots monitored by 2-DE proteomics

Sophie Alvarez; Ashley Galant; Joseph M. Jez; Leslie M. Hicks

doi:10.1002/pmic.201000450

Redox-regulatory mechanisms induced by oxidative stress in Brassica juncea roots monitored by 2-DE proteomics

Sophie Alvarez, Ashley Galant, Joseph M. Jez, Leslie M. Hicks

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

ROS, including hydrogen peroxide (H₂O₂), can serve as cellular signaling molecules following oxidative stress. Analysis of the redox state of proteins in Brassica juncea roots by 2-DE proteomics following treatment with either exogenous H₂O₂ or buthionine sulfoximine, which depletes glutathione to cause accumulation of endogenous H₂O₂, led to the identification of different sets of proteins. These data suggest that exogenous and endogenous oxidative stresses trigger specialized responses.

Original language	English (US)
Pages (from-to)	1346-1350
Number of pages	5
Journal	Proteomics
Volume	11
Issue number	7
DOIs	https://doi.org/10.1002/pmic.201000450
State	Published - Apr 2011
Externally published	Yes

Keywords

2-DE
Buthionine sulfoximine
Hydrogen peroxide
Oxidative stress
Plant proteomics

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1002/pmic.201000450

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title = "Redox-regulatory mechanisms induced by oxidative stress in Brassica juncea roots monitored by 2-DE proteomics",

abstract = "ROS, including hydrogen peroxide (H2O2), can serve as cellular signaling molecules following oxidative stress. Analysis of the redox state of proteins in Brassica juncea roots by 2-DE proteomics following treatment with either exogenous H2O2 or buthionine sulfoximine, which depletes glutathione to cause accumulation of endogenous H2O2, led to the identification of different sets of proteins. These data suggest that exogenous and endogenous oxidative stresses trigger specialized responses.",

keywords = "2-DE, Buthionine sulfoximine, Hydrogen peroxide, Oxidative stress, Plant proteomics",

author = "Sophie Alvarez and Ashley Galant and Jez, {Joseph M.} and Hicks, {Leslie M.}",

year = "2011",

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doi = "10.1002/pmic.201000450",

language = "English (US)",

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AU - Alvarez, Sophie

AU - Galant, Ashley

AU - Jez, Joseph M.

AU - Hicks, Leslie M.

PY - 2011/4

Y1 - 2011/4

N2 - ROS, including hydrogen peroxide (H2O2), can serve as cellular signaling molecules following oxidative stress. Analysis of the redox state of proteins in Brassica juncea roots by 2-DE proteomics following treatment with either exogenous H2O2 or buthionine sulfoximine, which depletes glutathione to cause accumulation of endogenous H2O2, led to the identification of different sets of proteins. These data suggest that exogenous and endogenous oxidative stresses trigger specialized responses.

AB - ROS, including hydrogen peroxide (H2O2), can serve as cellular signaling molecules following oxidative stress. Analysis of the redox state of proteins in Brassica juncea roots by 2-DE proteomics following treatment with either exogenous H2O2 or buthionine sulfoximine, which depletes glutathione to cause accumulation of endogenous H2O2, led to the identification of different sets of proteins. These data suggest that exogenous and endogenous oxidative stresses trigger specialized responses.

KW - 2-DE

KW - Buthionine sulfoximine

KW - Hydrogen peroxide

KW - Oxidative stress

KW - Plant proteomics

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JO - Proteomics

JF - Proteomics

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ER -

Redox-regulatory mechanisms induced by oxidative stress in Brassica juncea roots monitored by 2-DE proteomics

Abstract

Keywords

ASJC Scopus subject areas

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