TY - JOUR
T1 - Reduced PDEF expression increases invasion and expression of mesenchymal genes in prostate cancer cells
AU - Gu, Xuesong
AU - Zerbini, Luiz F.
AU - Otu, Hasan H.
AU - Bhasin, Manoj
AU - Yang, Quanli
AU - Joseph, Marie G.
AU - Grall, Franck
AU - Onatunde, Tomi
AU - Correa, Ricardo G.
AU - Libermann, Towia A.
PY - 2007/5/1
Y1 - 2007/5/1
N2 - The epithelium-specific Ets transcription factor, PDEF, plays a role in prostate and breast cancer, although its precise function has not been established. In prostate cancer, PDEF is involved in regulating prostate-specific antigen expression via interaction with the androgen receptor and NKX3.1, and down-regulation of PDEF by antiproliferative agents has been associated with reduced PDEF expression. We now report that reduced expression of PDEF leads to a morphologic change, increased migration and invasiveness in prostate cancer cells, reminiscent of transforming growth factor β (TGFβ) function and epithelial-to-mesenchyntal transition. Indeed, inhibition of PDEF expression triggers a transcriptional program of genes involved in the TGFβ pathway, migration, invasion, adhesion, and epithelial dedifferentiation. Our results establish PDEF as a critical regulator of genes involved in cell motility, invasion, and adhesion of prostate cancer cells.
AB - The epithelium-specific Ets transcription factor, PDEF, plays a role in prostate and breast cancer, although its precise function has not been established. In prostate cancer, PDEF is involved in regulating prostate-specific antigen expression via interaction with the androgen receptor and NKX3.1, and down-regulation of PDEF by antiproliferative agents has been associated with reduced PDEF expression. We now report that reduced expression of PDEF leads to a morphologic change, increased migration and invasiveness in prostate cancer cells, reminiscent of transforming growth factor β (TGFβ) function and epithelial-to-mesenchyntal transition. Indeed, inhibition of PDEF expression triggers a transcriptional program of genes involved in the TGFβ pathway, migration, invasion, adhesion, and epithelial dedifferentiation. Our results establish PDEF as a critical regulator of genes involved in cell motility, invasion, and adhesion of prostate cancer cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=34249295952&partnerID=8YFLogxK
U2 - 10.1158/0008-5472.CAN-06-3689
DO - 10.1158/0008-5472.CAN-06-3689
M3 - Article
C2 - 17483333
AN - SCOPUS:34249295952
SN - 0008-5472
VL - 67
SP - 4219
EP - 4226
JO - Cancer Research
JF - Cancer Research
IS - 9
ER -