Regenerated mdx mouse skeletal muscle shows differential mRNA expression

B. S. Tseng, P. Zhao, J. S. Pattison, S. E. Gordon, J. A. Granchelli, R. W. Madsen, L. C. Folk, E. P. Hoffman, F. W. Booth

Research output: Contribution to journalArticle

91 Scopus citations

Abstract

Despite over 3,000 articles published on dystrophin in the last 15 years, the reasons underlying the progression of the human disease, differential muscle involvement, and disparate phenotypes in different species are not understood. The present experiment employed a screen of 12,488 mRNAs in 16-wk-old mouse mdx muscle at a time when the skeletal muscle is avoiding severe dystrophic pathophysiology, despite the absence of a functional dystrophin protein. A number of transcripts whose levels differed between the mdx and human Duchenne muscular dystrophy were noted. A fourfold decrease in myostatin mRNA in the mdx muscle was noted. Differential upregulation of actin-related protein 2/3 (subunit 4), β-thymosin, calponin, mast cell chymase, and guanidinoacetate methyltransferase mRNA in the more benign mdx was also observed. Transcripts for oxidative and glycolytic enzymes in mdx muscle were not downregulated. These discrepancies could provide candidates for salvage pathways that maintain skeletal muscle integrity in the absence of a functional dystrophin protein in mdx skeletal muscle.

Original languageEnglish (US)
Pages (from-to)537-545
Number of pages9
JournalJournal of Applied Physiology
Volume93
Issue number2
DOIs
StatePublished - 2002

Keywords

  • Duchenne muscular dystrophy
  • Dystrophin
  • GeneChips
  • Microarrays

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Regenerated mdx mouse skeletal muscle shows differential mRNA expression'. Together they form a unique fingerprint.

  • Cite this

    Tseng, B. S., Zhao, P., Pattison, J. S., Gordon, S. E., Granchelli, J. A., Madsen, R. W., Folk, L. C., Hoffman, E. P., & Booth, F. W. (2002). Regenerated mdx mouse skeletal muscle shows differential mRNA expression. Journal of Applied Physiology, 93(2), 537-545. https://doi.org/10.1152/japplphysiol.00202.2002