Regional distribution and ionic requirement of Cl-/Ca2+-activated and Cl-/Ca2+-independent glutamate receptors in rat brain

Scott R. Whittemore; E. Edward Mena; Daniel T. Monaghan; Carl W. Cotman

doi:10.1016/0006-8993(83)90911-3

Regional distribution and ionic requirement of Cl^-/Ca²⁺-activated and Cl^-/Ca²⁺-independent glutamate receptors in rat brain

Scott R. Whittemore, E. Edward Mena, Daniel T. Monaghan, Carl W. Cotman

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Recent studies have shown that Cl^- and Ca²⁺ ions increase [³H]glutamate binding to rat forebrain synaptic plasma membranes by expressing a new class of glutamate receptors. We examined the regional distribution of these two classes of glutamate binding sites and further characterized their ionic requirements. Significant differences in both Cl^-/Ca²⁺-independent (basal) and Cl^-/Ca²⁺-activated receptors, as well as the ratios of these two receptor classes were observed among different areas of the CNS. Cl^- and Ca²⁺ appeared to act synergistically, with Cl^- ion an absolute requirement for Ca²⁺ stimulation, in expressing these additional binding sites. Ca²⁺ alone did not affect glutamate binding.

Original language	English (US)
Pages (from-to)	99-107
Number of pages	9
Journal	Brain Research
Volume	277
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(83)90911-3
State	Published - Oct 24 1983
Externally published	Yes

Keywords

2-amino-4-phosphonobutyrate
Cl/Ca-activated glutamate receptors
cerebellum
spinal cord

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

Access to Document

10.1016/0006-8993(83)90911-3

Cite this

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abstract = "Recent studies have shown that Cl- and Ca2+ ions increase [3H]glutamate binding to rat forebrain synaptic plasma membranes by expressing a new class of glutamate receptors. We examined the regional distribution of these two classes of glutamate binding sites and further characterized their ionic requirements. Significant differences in both Cl-/Ca2+-independent (basal) and Cl-/Ca2+-activated receptors, as well as the ratios of these two receptor classes were observed among different areas of the CNS. Cl- and Ca2+ appeared to act synergistically, with Cl- ion an absolute requirement for Ca2+ stimulation, in expressing these additional binding sites. Ca2+ alone did not affect glutamate binding.",

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