Abstract
We have examined the effect of differentiation on the expression of different members of the transforming growth factor type-beta (TGF-β) family using embryonal carcinoma (EC) cells and early mammalian embryos. We determined that TGF-β activity increases approximately 25-100% when the mouse EC cell line, F9, is induced to differentiate with retinoic acid (RA). Interestingly, the increased TGF-β activity reflects the induction of TGF-β2 secretion following differentiation of both F9 EC cells and the human EC cell line, NT2/D1. Using the technique of reverse transcription-polymerase chain reaction (RT-PCR), we have verified that differentiation induces the expression of TGF-β2 as well as a distant member of the TGF-β family, Vgr-1. Transcripts for TGF-β2 and Vgr-1 were readily detected in the differentiated cells of F9 and PC-13 but not in their undifferentiated counterparts. Moreover, TGF-β2 mRNA was readily detected in NT2/D1 cells following differentiation. In addition, transcripts for TGF-β2 were detected by RT-PCR in mouse morulae, preimplantation blastocysts and cultured blastocysts. Based on the data presented, it appears that the expression of both TGF-β2 and Vgr-1 is closely associated with the induction of differentiation during early development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 227-242 |
| Number of pages | 16 |
| Journal | Cytotechnology |
| Volume | 4 |
| Issue number | 3 |
| DOIs | |
| State | Published - Nov 1990 |
Keywords
- embryonal carcinoma cells
- mammalian embryogenesis
- polymerase chain reaction
- transforming growth factor type-beta
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering
- Clinical Biochemistry
- Cell Biology