Regulation of B cell tolerance by murine gangliosides

Harold C. Miller; William G. Chaney; Norman R. Klinman; Walter J. Esselman

doi:10.1016/0008-8749(82)90230-1

Regulation of B cell tolerance by murine gangliosides

Harold C. Miller, William G. Chaney, Norman R. Klinman, Walter J. Esselman

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Abstract

The potential role of gangliosides as modulators of the triggering of neonatal primary B lymphocytes at the single precursor cell level was evaluated. Tolerance was induced in splenic fragment cultures containing an excess of carrier-primed T cells. Gangliosides at low concentrations (20 ng/culture) abrogated the tolerogenic effect of haptens presented on carriers not recognized by environmental T cells. The permanent arrest of immature B-cell responsiveness resulting from tolerogen treatment was eliminated by the presence of gangliosides during tolerogen treatment. The active moiety in the glycolipid preparation which protected B cells during tolerogen treatment was separated by ion-exchange chromatography and demonstrated to be in the disialoganglioside fraction.

Original language	English (US)
Pages (from-to)	390-395
Number of pages	6
Journal	Cellular Immunology
Volume	67
Issue number	2
DOIs	https://doi.org/10.1016/0008-8749(82)90230-1
State	Published - Mar 1 1982
Externally published	Yes

ASJC Scopus subject areas

Immunology

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10.1016/0008-8749(82)90230-1

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@article{7f9da0dd0820484485b8b2132298c0f0,

title = "Regulation of B cell tolerance by murine gangliosides",

abstract = "The potential role of gangliosides as modulators of the triggering of neonatal primary B lymphocytes at the single precursor cell level was evaluated. Tolerance was induced in splenic fragment cultures containing an excess of carrier-primed T cells. Gangliosides at low concentrations (20 ng/culture) abrogated the tolerogenic effect of haptens presented on carriers not recognized by environmental T cells. The permanent arrest of immature B-cell responsiveness resulting from tolerogen treatment was eliminated by the presence of gangliosides during tolerogen treatment. The active moiety in the glycolipid preparation which protected B cells during tolerogen treatment was separated by ion-exchange chromatography and demonstrated to be in the disialoganglioside fraction.",

author = "Miller, {Harold C.} and Chaney, {William G.} and Klinman, {Norman R.} and Esselman, {Walter J.}",

note = "Funding Information: {\textquoteright} This work was supported by grants from the National Institutes of Health and National Cancer Institute (U.S. P.H.S. jCA13396, CAl5822, AI15797, A105678, CA09140, and CA24437 and American Cancer Society Grant CD-51A. HCM was the recipient of a American Cancer Society Faculty Research Award (FRA-147) and a Visiting Investigator in Immunology, Scripps Clinic and Research Foundation, La Jolla, California.",

year = "1982",

month = mar,

day = "1",

doi = "10.1016/0008-8749(82)90230-1",

language = "English (US)",

volume = "67",

pages = "390--395",

journal = "Cellular Immunology",

issn = "0008-8749",

publisher = "Academic Press Inc.",

number = "2",

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TY - JOUR

T1 - Regulation of B cell tolerance by murine gangliosides

AU - Miller, Harold C.

AU - Chaney, William G.

AU - Klinman, Norman R.

AU - Esselman, Walter J.

N1 - Funding Information: ’ This work was supported by grants from the National Institutes of Health and National Cancer Institute (U.S. P.H.S. jCA13396, CAl5822, AI15797, A105678, CA09140, and CA24437 and American Cancer Society Grant CD-51A. HCM was the recipient of a American Cancer Society Faculty Research Award (FRA-147) and a Visiting Investigator in Immunology, Scripps Clinic and Research Foundation, La Jolla, California.

PY - 1982/3/1

Y1 - 1982/3/1

N2 - The potential role of gangliosides as modulators of the triggering of neonatal primary B lymphocytes at the single precursor cell level was evaluated. Tolerance was induced in splenic fragment cultures containing an excess of carrier-primed T cells. Gangliosides at low concentrations (20 ng/culture) abrogated the tolerogenic effect of haptens presented on carriers not recognized by environmental T cells. The permanent arrest of immature B-cell responsiveness resulting from tolerogen treatment was eliminated by the presence of gangliosides during tolerogen treatment. The active moiety in the glycolipid preparation which protected B cells during tolerogen treatment was separated by ion-exchange chromatography and demonstrated to be in the disialoganglioside fraction.

AB - The potential role of gangliosides as modulators of the triggering of neonatal primary B lymphocytes at the single precursor cell level was evaluated. Tolerance was induced in splenic fragment cultures containing an excess of carrier-primed T cells. Gangliosides at low concentrations (20 ng/culture) abrogated the tolerogenic effect of haptens presented on carriers not recognized by environmental T cells. The permanent arrest of immature B-cell responsiveness resulting from tolerogen treatment was eliminated by the presence of gangliosides during tolerogen treatment. The active moiety in the glycolipid preparation which protected B cells during tolerogen treatment was separated by ion-exchange chromatography and demonstrated to be in the disialoganglioside fraction.

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U2 - 10.1016/0008-8749(82)90230-1

DO - 10.1016/0008-8749(82)90230-1

M3 - Article

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AN - SCOPUS:0020052328

SN - 0008-8749

VL - 67

SP - 390

EP - 395

JO - Cellular Immunology

JF - Cellular Immunology

IS - 2

ER -

Regulation of B cell tolerance by murine gangliosides

Abstract

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