Regulation of B cell tolerance by murine gangliosides

Harold C. Miller, William G. Chaney, Norman R. Klinman, Walter J. Esselman

Research output: Contribution to journalArticle

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Abstract

The potential role of gangliosides as modulators of the triggering of neonatal primary B lymphocytes at the single precursor cell level was evaluated. Tolerance was induced in splenic fragment cultures containing an excess of carrier-primed T cells. Gangliosides at low concentrations (20 ng/culture) abrogated the tolerogenic effect of haptens presented on carriers not recognized by environmental T cells. The permanent arrest of immature B-cell responsiveness resulting from tolerogen treatment was eliminated by the presence of gangliosides during tolerogen treatment. The active moiety in the glycolipid preparation which protected B cells during tolerogen treatment was separated by ion-exchange chromatography and demonstrated to be in the disialoganglioside fraction.

Original languageEnglish (US)
Pages (from-to)390-395
Number of pages6
JournalCellular Immunology
Volume67
Issue number2
DOIs
StatePublished - Mar 1 1982

ASJC Scopus subject areas

  • Immunology

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    Miller, H. C., Chaney, W. G., Klinman, N. R., & Esselman, W. J. (1982). Regulation of B cell tolerance by murine gangliosides. Cellular Immunology, 67(2), 390-395. https://doi.org/10.1016/0008-8749(82)90230-1