Regulation of carbohydrate metabolism in mouse liver. Effect of glucagon on gluconeogenic/glycolytic flux in isolated perfused livers

David E. Cook, Sheldon M. Schuster, Margaret L. Heidrick, Margery A. Fienhold, William Cook, Rod S. Markin

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

1. 1. Glucagon stimulated gluconeogenesis from both [U-14C]lactate and [14C]xylitol in isolated perfused mouse liver. 2. 2. Addition of cyclic AMP also stimulated gluconeogenesis from [U-14C]lactate. 3. 3. Glucagon caused a rapid (2.5 min) 12-fold increase in hepatic cyclic AMP but not cyclic GMP concentration. 4. 4. Glucagon caused a rapid and stable decrease in hepatic fructose 1,6-diphosphatase activity measured in vitro. 5. 5. The results are interpreted to indicate that glucagon stimulates hepatic gluconeogenesis in mice via cyclic AMP by two different mechanisms: (a) increased substrate uptake (i.e. utilization) and (b) increased gluconeogenic efficiency (i.e. inhibition of alternate substrate fates).

Original languageEnglish (US)
Pages (from-to)33-39
Number of pages7
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume64
Issue number1
DOIs
StatePublished - 1979

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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