TY - JOUR
T1 - Regulation of carbohydrate metabolism in mouse liver. Effect of glucagon on gluconeogenic/glycolytic flux in isolated perfused livers
AU - Cook, David E.
AU - Schuster, Sheldon M.
AU - Heidrick, Margaret L.
AU - Fienhold, Margery A.
AU - Cook, William
AU - Markin, Rod S.
N1 - Funding Information:
Acknowledgements--This work was supported in part by U.S. Public Health Service Research Grants AM 17400 and CA 16279. The authors acknowledge the excellent technical assistance of Laura Gaul.
PY - 1979
Y1 - 1979
N2 - 1. 1. Glucagon stimulated gluconeogenesis from both [U-14C]lactate and [14C]xylitol in isolated perfused mouse liver. 2. 2. Addition of cyclic AMP also stimulated gluconeogenesis from [U-14C]lactate. 3. 3. Glucagon caused a rapid (2.5 min) 12-fold increase in hepatic cyclic AMP but not cyclic GMP concentration. 4. 4. Glucagon caused a rapid and stable decrease in hepatic fructose 1,6-diphosphatase activity measured in vitro. 5. 5. The results are interpreted to indicate that glucagon stimulates hepatic gluconeogenesis in mice via cyclic AMP by two different mechanisms: (a) increased substrate uptake (i.e. utilization) and (b) increased gluconeogenic efficiency (i.e. inhibition of alternate substrate fates).
AB - 1. 1. Glucagon stimulated gluconeogenesis from both [U-14C]lactate and [14C]xylitol in isolated perfused mouse liver. 2. 2. Addition of cyclic AMP also stimulated gluconeogenesis from [U-14C]lactate. 3. 3. Glucagon caused a rapid (2.5 min) 12-fold increase in hepatic cyclic AMP but not cyclic GMP concentration. 4. 4. Glucagon caused a rapid and stable decrease in hepatic fructose 1,6-diphosphatase activity measured in vitro. 5. 5. The results are interpreted to indicate that glucagon stimulates hepatic gluconeogenesis in mice via cyclic AMP by two different mechanisms: (a) increased substrate uptake (i.e. utilization) and (b) increased gluconeogenic efficiency (i.e. inhibition of alternate substrate fates).
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U2 - 10.1016/0305-0491(79)90180-9
DO - 10.1016/0305-0491(79)90180-9
M3 - Article
C2 - 233788
AN - SCOPUS:0018713626
SN - 0305-0491
VL - 64
SP - 33
EP - 39
JO - Comparative Biochemistry and Physiology -- Part B: Biochemistry and
JF - Comparative Biochemistry and Physiology -- Part B: Biochemistry and
IS - 1
ER -