Regulation of major histocompatibility complex class II gene expression on bovine bronchial epithelial cells

John R. Spurzem, Olmero Sacco, Giovanni A. Rossi, Joe D. Beckmann, Stephen I. Rennard

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Induction and increased expression of major histocompatibility complex (MHC) class II antigens in tissues that do not normally express class II antigens is thought to be important in the pathogenesis of allograft rejection and may also be important in the pathogenesis of obliterative bronchiolitis in lung allografts. We hypothesized that MHC antigen expression on bronchial epithelial cells, particularly MHC class II expression, is regulated by immune mediators in a manner similar to the regulation of antigen expression by lymphoid cells. With an in vitro system of bovine bronchial epithelial cells, we evaluated the effects of both a lymphokine-containing supernatant and recombinant tumor necrosis factor-α on MHC class II expression. Immunofluorescence and flow cytometry demonstrated markedly increased MHC class II surface antigen expression on the bronchial epithelial cells after exposure to the lymphokine-containing supernatant. Tumor necrosis factor-α had no effect on MHC class II surface antigen expression. Immunosuppressant drugs altered the cellular responses to lymphokines in a manner similar to that of lymphoid cells. Dexamethasone suppressed the induction of MHC class II antigens at both the surface antigen and messenger RNA transcript levels. Cyclosporine did not have a consistent effect on the surface expression of class II antigens, but it did inhibit the messenger RNA transcript levels. These suppressive effects have implications for the therapy of airway disorders associated with lung and bone marrow transplantation.

Original languageEnglish (US)
Pages (from-to)94-102
Number of pages9
JournalThe Journal of Laboratory and Clinical Medicine
Volume120
Issue number1
StatePublished - Jul 1992

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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