Regulation of miRNA abundance by RNA binding protein TOUGH in Arabidopsis

Guodong Ren, Meng Xie, Yongchao Dou, Shuxin Zhang, Chi Zhang, Bin Yu

Research output: Contribution to journalArticlepeer-review

126 Scopus citations


MicroRNAs (miRNAs) are regulators of gene expression in plants and animals. The biogenesis of miRNAs is precisely controlled to secure normal development of organisms. Here we report that TOUGH (TGH) is a component of the DCL1-HYL1-SERRATE complex that processes primary transcripts of miRNAs [i.e., primary miRNAs (pri-miRNAs)] into miRNAs in Arabidopsis. Lack of TGH impairs multiple DCL activities in vitro and reduces the accumulation of miRNAs and siRNAs in vivo. TGH is an RNA-binding protein, binds pri-miRNAs and precursor miRNAs in vivo, and contributes to pri-miRNA-HYL1 interaction. These results indicate that TGH might regulate abundance of miRNAs through promoting DCL1 cleavage efficiency and/or recruitment of pri-miRNAs.

Original languageEnglish (US)
Pages (from-to)12817-12821
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number31
StatePublished - Jul 31 2012

ASJC Scopus subject areas

  • General

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