Regulation of surfactant protein D in the rodent prostate

Rebecca E. Oberley, Kelli L. Goss, Amado A. Quintar, Cristina A. Maldonado, Jeanne M. Snyder

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Background: Surfactant protein D (SP-D) is an innate immune protein that is present in mucosal lined surfaces throughout the human body, including the male reproductive tract. In the present study, we characterized the regulation of SP-D expression in the mouse and rat prostate. Methods: Real time reverse transcriptase polymerase chain reaction (RT-PCR) and immunostaining were used to characterize SP-D mRNA and protein in the mouse male reproductive tract. In order to evaluate the effects of testosterone on SP-D gene expression, we measured SP-D mRNA levels via real time RT-PCR in prostates from sham-castrated mice and castrated mice. In addition, we used a rat prostatitis model in which Escherichia coli was injected into the prostate in vivo to determine if infection influences SP-D protein levels in the prostate. Results: We found that SP-D mRNA and protein are present throughout the mouse male reproductive tract, including in the prostate. We determined that castration increases prostate SP-D mRNA levels (∼7 fold) when compared to levels in sham-castrated animals. Finally, we demonstrated that infection in the prostate results in a significant increase in SP-D content 24 and 48 hours post-infection. Conclusion: Our results suggestthat infection and androgens regulate SP-D in the prostate.

Original languageEnglish (US)
Article number42
JournalReproductive Biology and Endocrinology
Volume5
DOIs
StatePublished - Nov 7 2007

ASJC Scopus subject areas

  • Reproductive Medicine
  • Endocrinology
  • Obstetrics and Gynecology
  • Developmental Biology

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