Remodeling of chromatin under low intensity diffuse ultrasound

Sandra Noriega, Gaurav Budhiraja, Anuradha Subramanian

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A variety of mechanotransduction pathways mediate the response of fibroblasts or chondrocytes to ultrasound stimulation. In addition, regulatory pathways that co-ordinate stimulus-specific cellular responses are likely to exist. In this study, analysis was confined to the hypothesis that ultrasound stimulation (US) influences the chromatin structure, and that these changes may reflect a regulatory pathway that connects nuclear architecture, chromatin structure and gene expression. Murine fibroblasts seeded on tissue culture plates were stimulated with US (5.0 MHz (14 kPa), 51-s per application) and the thermal denaturation profiles of nuclei isolated from fibroblasts were assessed by dynamic scanning calorimetry (DSC). When compared to the thermal profiles obtained from the nuclei of non-stimulated cells, the nuclei obtained from stimulated cells showed a change in peak profiles and peak areas, which is indicative of chromatin remodeling. Independently, US was also observed to impact the histone (H1):chromatin association as measured indirectly by DAPI staining. Based on our work, it appears plausible that US can produce a remodeling of chromatin, thus triggering signal cascade and other intracellular mechanisms.

Original languageEnglish (US)
Pages (from-to)1331-1336
Number of pages6
JournalInternational Journal of Biochemistry and Cell Biology
Volume44
Issue number8
DOIs
StatePublished - Aug 2012

Keywords

  • Chromatin
  • Gene expression
  • Stimulation
  • Transition peaks
  • Ultrasound

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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