Removal of contaminating proteases from Bacillus thuringiensis parasporal crystals by density gradient centrifugation in NaBr

Kenneth W. Nickerson; Jane D. Swanson

doi:10.1007/BF00500100

Removal of contaminating proteases from Bacillus thuringiensis parasporal crystals by density gradient centrifugation in NaBr

Kenneth W. Nickerson, Jane D. Swanson

Biological Sciences

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Purification of the Bacillus thuringiensis protein crystal on NaBr density gradients confers a significant technical advantage in that the crystal-associated proteases are thereby removed. The use of protease-free crystals allows reliable determination of native crystal parameters.

Original language	English (US)
Pages (from-to)	213-215
Number of pages	3
Journal	European Journal of Applied Microbiology and Biotechnology
Volume	13
Issue number	4
DOIs	https://doi.org/10.1007/BF00500100
State	Published - Dec 1981

ASJC Scopus subject areas

Biotechnology
Microbiology

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@article{1031b5f5fed44a59bdd308feffc10938,

title = "Removal of contaminating proteases from Bacillus thuringiensis parasporal crystals by density gradient centrifugation in NaBr",

abstract = "Purification of the Bacillus thuringiensis protein crystal on NaBr density gradients confers a significant technical advantage in that the crystal-associated proteases are thereby removed. The use of protease-free crystals allows reliable determination of native crystal parameters.",

author = "Nickerson, {Kenneth W.} and Swanson, {Jane D.}",

year = "1981",

month = dec,

doi = "10.1007/BF00500100",

language = "English (US)",

volume = "13",

pages = "213--215",

journal = "European Journal of Applied Microbiology and Biotechnology",

issn = "0171-1741",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "4",

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TY - JOUR

T1 - Removal of contaminating proteases from Bacillus thuringiensis parasporal crystals by density gradient centrifugation in NaBr

AU - Nickerson, Kenneth W.

AU - Swanson, Jane D.

PY - 1981/12

Y1 - 1981/12

N2 - Purification of the Bacillus thuringiensis protein crystal on NaBr density gradients confers a significant technical advantage in that the crystal-associated proteases are thereby removed. The use of protease-free crystals allows reliable determination of native crystal parameters.

AB - Purification of the Bacillus thuringiensis protein crystal on NaBr density gradients confers a significant technical advantage in that the crystal-associated proteases are thereby removed. The use of protease-free crystals allows reliable determination of native crystal parameters.

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U2 - 10.1007/BF00500100

DO - 10.1007/BF00500100

M3 - Article

AN - SCOPUS:0019800682

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VL - 13

SP - 213

EP - 215

JO - European Journal of Applied Microbiology and Biotechnology

JF - European Journal of Applied Microbiology and Biotechnology

IS - 4

ER -

Removal of contaminating proteases from Bacillus thuringiensis parasporal crystals by density gradient centrifugation in NaBr

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