Removal of contaminating proteases from Bacillus thuringiensis parasporal crystals by density gradient centrifugation in NaBr

Kenneth W. Nickerson, Jane D. Swanson

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Purification of the Bacillus thuringiensis protein crystal on NaBr density gradients confers a significant technical advantage in that the crystal-associated proteases are thereby removed. The use of protease-free crystals allows reliable determination of native crystal parameters.

Original languageEnglish (US)
Pages (from-to)213-215
Number of pages3
JournalEuropean Journal of Applied Microbiology and Biotechnology
Volume13
Issue number4
DOIs
StatePublished - Dec 1981

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Fingerprint Dive into the research topics of 'Removal of contaminating proteases from Bacillus thuringiensis parasporal crystals by density gradient centrifugation in NaBr'. Together they form a unique fingerprint.

Cite this