Abstract
Background: DNA with two sites of damage in close proximity might not be repaired as efficiently as DNA with a single damage site. Materials and Methods: To study this hypothesis, we utilized short oligodeoxyribonucleotides with a stable adduct 7 or 16 nucleotides (nt) downstream from an apurinic (AP) site. Repair by extracts of human breast epithelial MCF-10A1 cells was assayed by quantifying the incorporation of [α-32P]dTTP. Results: The level of repair of an oligodeoxyribonucleotide with an AP site 7 nt from a stable adduct was comparable to that of the oligodeoxyribonucleotide with only an AP site. A decrease in overall repair of oligodeoxyribonucleotides containing an AP site and a stable adduct was observed if these lesions were 16 nt apart compared to the presence of only an AP site. Conclusion: The ability of human breast MCF-10A1 cells to repair DNA adducts and AP sites is affected by other near-by lesions.
Original language | English (US) |
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Pages (from-to) | 117-121 |
Number of pages | 5 |
Journal | Anticancer Research |
Volume | 24 |
Issue number | 1 |
State | Published - Jan 2004 |
Keywords
- Apurinic sites
- Base excision repair
- DNA adducts
- DNA repair
- Human breast cell extracts
- Nucleotide excision repair
ASJC Scopus subject areas
- Oncology
- Cancer Research