Replication of coxsackievirus B3 in primary cell cultures generates novel viral genome deletions

K. S. Kim, N. M. Chapman, S. Tracy

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Coxsackievirus B3 (CVB3) generates 5′-terminally deleted genomes (TDs) during replication in murine hearts. We show here that CVB3 populations with TDs can also be generated within two to three passages of CVB3 in primary, but not immortalized, cell cultures. Deletions of less than 49 nucleotides increase in size during passage, while 5′ TDs of 49 nucleotides appear to be the maximum deletion size. The cellular environment of contact-inhibited primary cell cultures or the myocardium in vivo is sufficient for the selection of 5′ TDs over undeleted genomes.

Original languageEnglish (US)
Pages (from-to)2033-2037
Number of pages5
JournalJournal of virology
Volume82
Issue number4
DOIs
StatePublished - Feb 2008

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Fingerprint

Dive into the research topics of 'Replication of coxsackievirus B3 in primary cell cultures generates novel viral genome deletions'. Together they form a unique fingerprint.

Cite this