@article{05651b65e83f40a29952eed3d28c368f,
title = "Resistome and mobilome in surface runoff from manured soil as affected by setback distance",
abstract = "Land application of livestock manure introduces antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) into the soil environment. The objectives of this study were to examine the changes of resistome and mobilome in runoff and soil as a function of setback distance, i.e., the distance between manured soil and surface water, and to quantify the contributions of manure and background soil to the ARGs and MGEs in surface runoff. The resistome and mobilome in runoff and soil from a field-scale plot study were characterized using a high throughput quantitative polymerase chain reaction (HT-qPCR) array. It was estimated that a setback distance of ~40 m is required to reduce the total abundance of ARGs and MGEs in runoff from amended plots to that in control runoff. The resistome and mobilome of the soil in the setback region was not affected by manure-borne ARGs and MGEs. SourceTracker analyses revealed that background soil gradually became the predominant source of the ARGs and MGEs in runoff as setback distance increased. The results demonstrate how manure-borne ARGs and MGEs dissipated in agricultural runoff with increasing setback distance and had limited impacts on the resistome and mobilome of soil within the setback region.",
keywords = "Resistome, Runoff, Setback distance, Soil, Swine manure",
author = "Mware, {Noelle A.} and Hall, {Maria C.} and Selvakumar Rajendran and Gilley, {John E.} and Schmidt, {Amy M.} and Bartelt-Hunt, {Shannon L.} and Yifan Zhang and Xu Li",
note = "Funding Information: The study was funded by the National Pork Board (#16-072) and the US Department of Agriculture (#2017-68003-26497). N.A.M. and M.C.H were partially supported by the Daugherty Water for Food Global Institute. S.K. was supported by the WARI program through the Daugherty Water for Food Global Institute and Indo-US Science and Technology Forum. The authors also thank Robert Stedtfeld for technical assistance. The novelty of the work lies in three aspects. First, while livestock manure has been recognized as a source of antibiotic resistance genes (ARGs) to the environment, there has been few field studies to quantify the transport of manure-borne ARGs in agricultural runoff. We employed field plots and a rainfall simulator in this work. Second, instead of quantitative PCR, we employed high-throughput quantitative PCR (HT-qPCR) to study the entire resistome and mobilome of the runoff and soil samples. Third, the data from HT-qPCR was processed using a Bayesian model to distinguish manure-borne ARGs from background soil-borne ARGs. Funding Information: The study was funded by the National Pork Board ( #16-072 ) and the US Department of Agriculture ( #2017-68003-26497 ). N.A.M. and M.C.H were partially supported by the Daugherty Water for Food Global Institute. S.K. was supported by the WARI program through the Daugherty Water for Food Global Institute and Indo-US Science and Technology Forum. The authors also thank Robert Stedtfeld for technical assistance. Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",
year = "2022",
month = may,
day = "5",
doi = "10.1016/j.jhazmat.2022.128278",
language = "English (US)",
volume = "429",
journal = "Journal of Hazardous Materials",
issn = "0304-3894",
publisher = "Elsevier",
}