Resonance Raman Spectra for Flavin Derivatives Modified in the 8 Position

Lawrence M. Schopfer, Jeanne P. Haushalter, Michael Smith, Magdy Milad, Michael D. Morris

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20 Scopus citations

Abstract

Spontaneous resonance Raman or resonance-enhanced AC-coupled inverse Raman spectra were obtained for 8-mercaptoriboflavin, 8-(dimethylamino)riboflavin, 8- hydroxyriboflavin, and 8-aminoriboflavin all free in solution. These Raman spectra were all similar to one another and markedly different from that of riboflavin. In addition, the Raman spectra of 8-mercaptoflavin bound to the apoproteins of old yellow enzyme, glucose oxidase, and L-lactate oxidase were determined. The Raman spectra of these protein-bound flavins were distinctly different from both those of the above 8-substituted riboflavins and that of riboflavin. An argument is presented in favor of assigning a quinonoid electronic structure to these protein-bound flavins.

Original languageEnglish (US)
Pages (from-to)6734-6739
Number of pages6
JournalBiochemistry
Volume20
Issue number23
DOIs
StatePublished - Nov 1981

ASJC Scopus subject areas

  • Biochemistry

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    Schopfer, L. M., Haushalter, J. P., Smith, M., Milad, M., & Morris, M. D. (1981). Resonance Raman Spectra for Flavin Derivatives Modified in the 8 Position. Biochemistry, 20(23), 6734-6739. https://doi.org/10.1021/bi00526a032